Kiko Hoʻokaʻawale DNA Tissue Nā Kiko Hoʻoemi DNA mai nā Laʻana Tissue holoholona
Nā wehewehe kime
Hoʻohana kēia pahu i kahi kolamu DNA-wale nō i hiki ke hoʻopaʻa pono i ka DNA, Foregene protease a me kahi ʻōnaehana pale kūʻokoʻa.Hiki ke kiʻi ʻia ka DNA genomic kiʻekiʻe mai nā cell moʻomeheu like ʻole a me nā ʻiʻo holoholona i loko o 30 a 50 mau minuke.
ʻO ka membrane silica gel DNA wale nō i hoʻohana ʻia i loko o ke kolamu milo, ʻo ia ka mea hou kūikawā a Foregene, hiki ke hoʻopaʻa pono a paʻa pono i ka DNA, a hoʻonui i ka lawe ʻia ʻana o ka RNA, nā protein haumia, nā ion a me nā mea hoʻohui ʻē aʻe i loko o nā cell.Hiki ke hoʻomaʻemaʻe ʻia ka DNA genomic kiʻekiʻe 5-80μg mai 10-50mg kiko.
Nā kikoʻī
Kiko Hoʻokaʻawale DNA Tissue holoholona | |||
Kiko maʻiʻo | DE-05011 | DE-05012 | DE-05013 |
50T | 100T | 250T | |
Hoʻopaʻa L1 | 20ml | 40ml | 100ml |
Mālama L2* | 20ml | 40ml | 100ml |
Hoʻopaʻa PW* | 25ml | 50ml | 125ml |
Hoʻopaʻa WB | 25ml | 50ml | 125ml |
Hoʻopaʻa EB | 10ml | 20ml | 50ml |
Foregene Protease | 1.25ml | 2.5ml | 6.5ml |
DNA-wale wale ke kolamu | 50 | 100 | 250 |
Manual | 1 | 1 | 1 |
*: Loaʻa i ka Buffer L2 a me ka Buffer PW ka paʻakai paʻakai hoʻonāukiuki.E ʻoluʻolu e ʻaʻahu i nā mīkina lima a e hana i nā mea palekana i ka wā e hana ai.
Nā hiʻohiʻona & pono
■ ʻAʻole hoʻohaumia ʻo RNase: ʻO ka DNA-Only Column i hāʻawi ʻia i loko o ka pahu e hiki ai ke hoʻoneʻe i ka RNA mai ka DNA genomic me ka ʻole o ka RNase hou i ka wā o ka hoʻokolohua, no laila e pale ai i ka hale hana mai ka haumia ʻana e ka RNase exogenous.
■ ʻO ka wikiwiki wikiwiki: He ʻoi aku ka ʻoihana o Foregene Protease ma mua o nā protease like a ʻoi aku ka wikiwiki o ka hoʻoemi ʻana i nā laʻana.
■ Maʻalahi : hiki ke hoʻopau ʻia ka hana unuhi DNA genomic i 50 mau minuke.
■ Maikaʻi: Hana ʻia ka centrifugation ma ka lumi wela, ʻaʻole koi ʻia ka centrifugation haʻahaʻa haʻahaʻa haʻahaʻa a i ʻole ethanol precipitation o DNA.
■ Palekana: ʻaʻole koi ʻia ka reagent organik.
■ Kiʻekiʻe kiʻekiʻe: ʻO ka DNA genomic i unuhi ʻia he mau ʻāpana nui, ʻaʻohe RNA, ʻaʻohe RNase, a haʻahaʻa haʻahaʻa haʻahaʻa, hiki ke hoʻokō i nā koi o nā hoʻokolohua like ʻole.
Ke noi pahu
He kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka DNA genomic mai nā ʻano mea kanu hou a i ʻole ka hau a me nā ʻiʻo holoholona.
Ka holo hana
Diagram
Ka mālama ʻana a me ka mālama ʻana
Hiki ke mālama ʻia ka pahu no 12 mau mahina ma ke ana wela (15–25 ℃) a me 2–8 ℃ no ka manawa lōʻihi.