ʻO BCL6 ʻĀpana Wehe ʻAi ʻAi ʻelua
ʻO ka wehewehe ʻana o ka pahu:
Ma muli o ke kumu o ka hoʻohui pū ʻana o nā kumu DNA, ua hoʻohana ʻia ka ʻimi ʻalani BCL6 a me ka ʻimi ʻōmaʻomaʻo BCL6 e hoʻohui pū me ke kaʻina pahuhopu DNA i loko o ka nucleus, a ua nānā ʻia ka ʻike kūlana gene i loko o ka nucleus ma lalo o ka microscope fluorescence.
wehewehe
Ma muli o ke kumu o ka hoʻohui pū ʻana o nā kumu DNA, ua hoʻohana ʻia ka ʻimi ʻalani BCL6 a me ka ʻimi ʻōmaʻomaʻo BCL6 e hoʻohui pū me ke kaʻina pahuhopu DNA i loko o ka nucleus, a ua nānā ʻia ka ʻike kūlana gene i loko o ka nucleus ma lalo o ka microscope fluorescence.
Nā ʻāpana pahu
| ʻāpana | Nā kikoʻī | ||
| 5 Hoao | 10 Hoao | 20 Hoao | |
| ʻO BCL6 ʻĀpana Wehe ʻAi ʻAi ʻelua | 50μl | 100μl | 200μl |
Ke noi pahu
Hoʻohana ʻia kēia kit e ʻike pono i ke ʻano o ke ʻano BCL6 kanaka i loko o nā laʻana cytological a i ʻole nā palapala kikoo paraffin-embedded in vitro.
Nā koi hoʻohālike
-Kiʻi mea hoʻohālike
Ua hoʻopaʻa ʻia nā ʻano kikoo paraffin i hoʻopaʻa ʻia i loko o 10% neutral buffered formalin, a ʻo ka mānoanoa ʻāpana ma waena o 3 a me 5 µm.
-Kelepona mea hoʻohālike
No ka iwi iwi a i ʻole nā ʻāpana koko ʻokoʻa, mālama ʻia nā laʻana hou i hoʻopaʻa ʻole ʻia ma 4 ℃ no nā hola he 24, a mālama ʻia ka hoʻokuʻu ʻana o ka cell paʻa ma-20 ℃.
Ka mālama ʻana a me ka mālama ʻana
Mālama ʻia ma -20 ℃ ± 5 ℃ i ka pōʻeleʻele, kūpono no 12 mau mahina.ʻO ka halihali ma lalo o 8 ℃.
