TP53/CSP17 ʻAiʻoniʻoni Kaulua
ʻO ka wehewehe ʻana o ka pahu:
E like me ke kumu o ka DNA base complementary pairing, ua hoʻohana ʻia ka probe ʻalani-ʻulaʻula TP53 a me ka ʻimi ʻōmaʻomaʻo CSP17 e hoʻohui pū me ke kaʻina pahuhopu DNA i loko o ka nucleus, a ua nānā ʻia ka ʻike mokuʻāina i loko o ka nucleus a nānā ʻia ma lalo o kahi microscope fluorescence.
wehewehe
E like me ke kumu o ka DNA base complementary pairing, ua hoʻohana ʻia ka probe ʻalani-ʻulaʻula TP53 a me ka ʻimi ʻōmaʻomaʻo CSP17 e hoʻohui pū me ke kaʻina pahuhopu DNA i loko o ka nucleus, a ua nānā ʻia ka ʻike mokuʻāina i loko o ka nucleus a nānā ʻia ma lalo o kahi microscope fluorescence.
Nā ʻāpana pahu
| ʻāpana | Nā kikoʻī | ||
| 5 Hoao | 10 Hoao | 20 Hoao | |
| TP53/CSP17 ʻAiʻoniʻoni Kaulua | 50μl | 100μl | 200μl |
Ke noi pahu
Hoʻohana ʻia kēia kit e ʻike pono i ka ʻino o ke ʻano TP53 kanaka i loko o nā laʻana cytological a i ʻole nā palapala kikoo paraffin-embedded in vitro.
Nā koi hoʻohālike
- Kiʻi kiko
Ua hoʻopaʻa ʻia nā ʻano kikoo paraffin i hoʻopaʻa ʻia i loko o 10% neutral buffered formalin, a ʻo ka mānoanoa ʻāpana ma waena o 3 a me 5 µm.
- Hoʻohālikelike kelepona
No ka iwi iwi a i ʻole nā laʻana koko ʻaoʻao, pono e mālama ʻia nā laʻana hou i hoʻopaʻa ʻole ʻia ma 4 ° C no nā hola he 24, a pono e mālama ʻia ka hoʻokuʻu ʻana o ke kelepona paʻa ma -20°C.
Ka mālama ʻana a me ka mālama ʻana
Mālama ʻia ma -20 ℃ ± 5 ℃ i ka pōʻeleʻele, kūpono no 12 mau mahina.ʻO ka halihali ma lalo o 8 ℃.
