He mea hana akamai mākou.Ke lanakila nei i ka hapa nui mai nā palapala hōʻoia koʻikoʻi o kāna mākeke no ke kumu kūʻai ʻoi loa ʻo Kina Lab 64 Well Plate Magnetic Bead Nucleic Acid ExtractionDNA Rna Hoomaemae Isolation Kitme CE, Me kā mākou hoʻoikaika ʻana, ua lanakila kā mākou huahana i ka hilinaʻi o nā mea kūʻai aku a hiki ke kūʻai ʻia ma ʻaneʻi a ma waho.
He mea hana akamai mākou.Ke lanakila nei i ka hapa nui mai nā palapala hōʻoia koʻikoʻi o kāna mākeke noKina Nucleic Acid Extraction Kit, DNA Rna Hoomaemae Isolation Kit, Makemake mākou e kono i nā mea kūʻai mai nā ʻāina ʻē e kūkākūkā i ka ʻoihana me mākou.Hiki iā mākou ke hōʻike i kā mākou mea kūʻai aku me nā huahana kiʻekiʻe a me ka lawelawe maikaʻi loa.Manaʻo mākou e loaʻa iā mākou nā pilina pili maikaʻi a hana i ka wā e hiki mai ana no nā ʻaoʻao ʻelua.

Nā kikoʻī

50 Mākaukau, 200 Mākaukau

Hoʻohana ka kit i ke kolamu spin a me ka formula i hoʻomohala ʻia e Foregene, hiki iā ia ke hoʻokaʻawale maikaʻi i ka RNA holoʻokoʻa kiʻekiʻe a kiʻekiʻe mai nā ʻiʻo mea kanu me nā polysaccharides haʻahaʻa a me nā polyphenols.No nā laʻana mea kanu me nā polysaccharides kiʻekiʻe a i ʻole polyphenols, pono e hoʻohana i ka Plant Total RNA Isolation Plus Kit no ka loaʻa ʻana o nā hopena unuhi RNA maikaʻi aʻe.Hāʻawi ka pahu i ke kolamu hoʻomaʻemaʻe DNA e hiki ke wehe maʻalahi i ka DNA genomic mai ka supernatant a me ka lysate kiko.Hiki i ke kolamu RNA-wale ke hoopaa pono i ka RNA.Hiki i ke kit ke hana i ka nui o nā laʻana i ka manawa like.

ʻAʻole i loko o ka ʻōnaehana holoʻokoʻa RNase, no laila ʻaʻole e hoʻohaʻahaʻa ʻia ka RNA i hoʻomaʻemaʻe ʻia.Hiki i ka Buffer PRW1 a me ka Buffer PRW2 ke hōʻoia ʻaʻole i haumia ka RNA i loaʻa e ka protein, DNA, nā ion, a me nā pūhui organik.

Nā ʻāpana pahu

Nā hiʻohiʻona & pono

■ Ka hana ma ka lumi wela (15-25 ℃) ma ke kaʻina holoʻokoʻa, me ka ʻauʻau hau a me ka centrifugation haʻahaʻa wela.
■ Hoʻopiha piha RNase-Free, ʻaʻohe pono e hopohopo e pili ana i ka hōʻino ʻana o RNA.
■ Hoʻopili pono ʻia ke kolamu hoʻomaʻemaʻe DNA i ka DNA, i hiki ai i ka pahu ke hoʻoneʻe i ka ʻino DNA genomic me ka hoʻohui ʻole ʻana i ka DNase.
■ Loaʻa RNA kiʻekiʻe: Hiki i ke kolamu RNA-wale a me ka hoʻohālikelike kū hoʻokahi ke hoʻomaʻemaʻe pono i ka RNA.
■ ʻO ka wikiwiki wikiwiki: maʻalahi e hana a hiki ke hoʻopau i loko o 30 mau minuke.
■ Palekana: ʻaʻole koi ʻia ka reagent organik.
■ Kiʻekiʻe: ʻO nā ʻāpana RNA i hoʻomaʻemaʻe ʻia he maʻemaʻe kiʻekiʻe, ʻaʻohe protein a me nā mea haumia ʻē aʻe, a hiki ke hālāwai me nā noi hoʻokolohua like ʻole.

Ke noi pahu

He kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka RNA a pau mai nā laʻana o nā mea kanu hou a i ʻole ka hau (ʻoi aku ka ʻiʻo o ka lau mea kanu hou) me ka polysaccharide haʻahaʻa a me ka polyphenol content.

Ka holo hana

Diagram

ʻO ka Plant Total RNA Isolation Kit Plus i hana ʻia he 50mg o nā lau hou o polysaccharides a me polyphenols, a ua hoʻāʻo ʻia ʻo 5% RNA i hoʻomaʻemaʻe ʻia e ka electrophoresis.
1: Maiʻa
2: Ginkgo
3: pulupulu
4: Pomegerane

Ka mālama ʻana a me ka mālama ʻana

Hiki ke mālama ʻia ka pahu no 12 mau mahina ma ka lumi wela (15–25 ℃) ma kahi maloʻo, a me 2–8 ℃ no ka manawa lōʻihi (24 mau mahina).

Hiki ke mālama ʻia ka Buffer PSL1 ma 4 ℃ no 1 mahina ma hope o ka hoʻohui ʻana i ka 2-hydroxy-1-ethanethiol (koho).ʻO ka lanakila ʻana i ka hapa nui mai nā palapala hōʻoia koʻikoʻi o kāna mākeke no ke kumukūʻai ʻoi loa ʻo Kina Lab 64 Well Plate Magnetic Bead Nucleic Acid Extraction DNA Rna Purification Isolation Kit me CE, Me kā mākou hoʻoikaika ʻana, ua lanakila kā mākou huahana i ka hilinaʻi o nā mea kūʻai aku a kūʻai nui ʻia ma ʻaneʻi a ma waho.
Kumukuai Mimi loaKina Nucleic Acid Extraction Kit, DNA Rna Purification Isolation Kit, Makemake mākou e kono i nā mea kūʻai mai nā ʻāina ʻē e kūkākūkā i ka ʻoihana me mākou.Hiki iā mākou ke hōʻike i kā mākou mea kūʻai aku me nā huahana kiʻekiʻe a me ka lawelawe maikaʻi loa.Manaʻo mākou e loaʻa iā mākou nā pilina pili maikaʻi a hana i ka wā e hiki mai ana no nā ʻaoʻao ʻelua.