ʻO nā mea a pau a mākou e hana ai e pili pū me kā mākou kumu "Consumer initial, hilinaʻi i ka 1st, e hoʻolaʻa ana a puni ka meaʻai meaʻai a me ka palekana o ke kaiapuni no ka High Performance China Magpure Viral Nucleic Acid Isolation Kit me Preassembly 96 Well Plate, ʻike ʻia kā mākou kumu i kēlā me kēia manawa: e hāʻawi i ka hopena maikaʻi maikaʻi i ka helu hoʻokūkū i nā mea kūʻai aku ma ka honua.Hoʻokipa mākou i nā mea kūʻai aku e kāhea iā mākou no nā kauoha OEM a me ODM.
ʻO nā mea a pau a mākou e hana ai e pili pū me kā mākou kumu "Consumer initial, hilinaʻi ma ka 1st, e hoʻolaʻa ana a puni ka meaʻai meaʻai a me ka palekana o ke kaiapuni noKina Rna/DNA Unuhi/Hoomaemae, Viral Rna Isolation, I loko o 11 mau makahiki, Ua komo mākou ma mua o 20 mau hōʻikeʻike, loaʻa ka mahalo kiʻekiʻe loa mai kēlā me kēia mea kūʻai.Manaʻo mau kā mākou hui e hāʻawi i ka mea kūʻai aku i nā huahana maikaʻi loa me ke kumu kūʻai haʻahaʻa.Ke hana nui nei mākou e hoʻokō i kēia kūlana win-win a hoʻokipa maikaʻi iā ʻoe e hui pū me mākou.E hui pū me mākou, e hōʻike i kou nani.E lilo mau mākou i kāu koho mua.E hilinaʻi iā mākou, ʻaʻole ʻoe e pau ka naʻau.

Nā wehewehe kits

50 Mākaukau, 200 Mākaukau

Ke hoʻohana nei kēia kit i ka kolamu spin a me ka formula i hoʻomohala ʻia e kā mākou hui, hiki ke unuhi i ka RNA holoʻokoʻa kiʻekiʻe-maʻemaʻe a kiʻekiʻe loa mai nā ʻano holoholona like ʻole me ka hana kiʻekiʻe.Hiki i ke kolamu RNA-wale ke hoʻopaʻa maikaʻi i ka RNA a hiki ke hana ʻia i ka manawa like me kahi ʻano ʻokoʻa He nui nā laʻana.

ʻO ka pūnaewele holoʻokoʻa he RNase-Free, iʻole e hoʻohaʻahaʻaʻia ka RNA i unuhiʻia;Buffer RW1, Buffer RW2 ʻōnaehana holoi holoi, no laila ʻo ka RNA i loaʻa ʻole i ka protein, DNA, ion, a me ka pollution hui.

Nā ʻāpana pahu:

Nā hiʻohiʻona & pono

■ ʻAʻole pono e hopohopo no ka hoʻohaʻahaʻa ʻana o RNA;ʻo RNase-Free ka ʻōnaehana holoʻokoʻa
■ Hoʻopau maikaʻi i ka DNA-hoʻohana i ka DNA-Cleaning Column
■ Wehe i ka DNA me ka hookomo ole i ka DNase
■ Maalahi-ua pau na hana a pau ma ka wela lumi
■ Hiki ke hoʻopau i ka hana wikiwiki i 30 mau minuke
■ Palekana-ʻaʻole pono ka mea hoʻoheheʻe organik
■ Maʻemaʻe kiʻekiʻe -OD260/280≈1.8-2.1

Ke noi pahu

He kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka RNA holoʻokoʻa mai nā ʻano ʻiʻo holoholona hou a hauʻoli paha a i ʻole nā ​​​​pūnaewele moʻomeheu.

Nā palena huahana

■ Nā noi i lalo: ka hoʻohui ʻana o ka cDNA mua, RT-PCR, molecular cloning, Northern Blot, etc.
■ Nā laʻana: nā ʻiʻo holoholona, ​​nā pūnaewele moʻomeheu
■ Ka Papa Hana: Tissues 10-20mg, Cells(2-5)×10⁶
■ Ka hiki ke hoʻopaʻa DNA kiʻekiʻe o ke kolamu hoʻomaʻemaʻe: 80 μg
■ Ka nui elution: 50-200 μl

Ka holo hana

Diagram

ʻO ka Animal Total RNA Isolation Kit i mālama ʻia he 20mg
Nā laʻana ʻiole hou, e lawe i ka 5% i hoʻomaʻemaʻe ʻia ka RNA 1% agar

ʻO ka electrophoresis glycogel
1: Spleen 2: Kidney
3: Ake 4: Puuwai

Ka mālama ʻana a me ka mālama ʻana

Hiki ke mālama ʻia ka pahu no 24 mau mahina ma ka lumi wela (15–25 ℃) a i ʻole 2–8 ℃ no ka manawa lōʻihi.Hiki ke mālama ʻia ʻo Buffer RL1 ma 4 ℃ no 1 mahina ma hope o ka hoʻohui ʻana i ka β- mercaptoethanol (koho). ʻO nā mea a pau a mākou e hana ai e pili pū me kā mākou kumu "Consumer initial, Rely on 1st, devoting around the food stuff packaging and environmental safety for High Performance China Magpure Viral Nucleic Acid Isolation Kit me Preassembly 96 ka maikaʻi o ka mea kūʻai aku i ka maikaʻi o ka mea kūʻai aku. ka honua.Hoʻokipa mākou i nā mea kūʻai aku e kāhea iā mākou no nā kauoha OEM a me ODM.
Hana KiʻekiʻeKina Rna/DNA Unuhi/Hoomaemae, Viral Rna Isolation, I loko o 11 mau makahiki, Ua komo mākou ma mua o 20 mau hōʻikeʻike, loaʻa ka mahalo kiʻekiʻe loa mai kēlā me kēia mea kūʻai.Manaʻo mau kā mākou hui e hāʻawi i ka mea kūʻai aku i nā huahana maikaʻi loa me ke kumu kūʻai haʻahaʻa.Ke hana nui nei mākou e hoʻokō i kēia kūlana win-win a hoʻokipa maikaʻi iā ʻoe e hui pū me mākou.E hui pū me mākou, e hōʻike i kou nani.E lilo mau mākou i kāu koho mua.E hilinaʻi iā mākou, ʻaʻole ʻoe e pau ka naʻau.

ʻAʻole lawe ʻia ka RNA a i ʻole nā ​​​​hua RNA

Nui nā ʻano like ʻole e pili ana i ka pono o ka hoʻihoʻi hou ʻana, e like me: kiko kiko RNA maʻiʻo, ke ʻano o ka hana, ka nui elution, etc.

1. Hana ʻia ka ʻauʻau hau a i ʻole cryogenic (4 °C) centrifugation i ka wā o ka hana.

Manaʻo: E hana ma ka lumi wela (15-25 ° C) i ke kaʻina holoʻokoʻa, mai ʻauʻau hau a centrifuge i nā haʻahaʻa haʻahaʻa.

2. Ka mālama pono ʻole ʻana i ka laʻana a i ʻole ka nui o ka manawa e mālama ai i ka laʻana.

Manaʻo Manaʻo: E kūʻai i nā laʻana ma -80 °C a i ʻole i loko o ka hau hau i loko o ka wai hau a pale i ka hoʻohana pinepine ʻana i ka hau hau;e ho'āʻo e hoʻohana i ka ʻiʻo hou a i ʻole nā ​​​​pūnaewele moʻomeheu no ka unuhi ʻana i ka RNA.

3. lawa ole la'ana lysis.

Manaʻo Manaʻo: I ka wā e hoʻohui ai i ka ʻiʻo, e hōʻoia i ka hoʻomaʻemaʻe ʻia ʻana o ka ʻiʻo a ua hoʻokaʻawale ʻia nā mea kikoo e wehewehe i ka hoʻokuʻu ʻana o RNA.

4. ʻAʻole i hoʻohui pono ʻia ka eluent.

Manaʻo: E hōʻoia i ka RNase-Free ddH₂Hoʻohui ʻia ʻo O i kahi dropwise i ka waena o ka membrane kolamu hoʻomaʻemaʻe.

5. ʻAʻole i hoʻohui ʻia ka leo kūpono o ka ethanol piha i ka Buffer RL2 a i ʻole Buffer RW2.

Manaʻo Manaʻo: E hahai i nā kuhikuhi, e hoʻohui i ka leo kūpono o ka ethanol piha i ka Buffer RL2 a me Buffer RW2 a hui maikaʻi ma mua o ka hoʻohana ʻana i ka pahu.

6. ʻAʻole kūpono ke ʻano o ka laʻana.

Manaʻo: E hoʻohana i ka 10-20 mg o ka ʻiʻo a i ʻole (1-5) × 10⁶nā pūnaewele no 500 μl buffer RL1, no ka mea, hiki i ka hoʻohana ʻana i ka ʻiʻo nui ke hopena i ka hōʻemi ʻana i ka RNA extraction.

7. Ka nui elution kūpono ʻole a i ʻole ka elution piha ʻole.

Manaʻo: ʻO ka nui elution o ke kolamu hoʻomaʻemaʻe ʻo 50-200 μl;inā ʻaʻole ʻoluʻolu ka hopena elution, pono ia e hoʻolōʻihi i ka manawa kau wela o ka lumi ma hope o ka hoʻohui ʻana i ka RNase-Free ddH preheated.₂ʻO, e laʻa no 5-10 min.

8. He koena ethanol ka kolamu hoʻomaʻemaʻe ma hope o ka holoi ʻana o Buffer RW2.

Manaʻo Manaʻo: Inā loaʻa ke koena ethanol ma hope o ka holoi ʻana o Buffer RW2, ʻo ka centrifugation paipu ʻole no 1min, hiki ke hoʻonui ʻia ka manawa no ka hana centrifugation pahu ʻole i 2min, a i ʻole ke kolamu hoʻomaʻemaʻe hiki ke kau ʻia ma ka lumi wela no 5 min e wehe pono i ke koena ethanol.

Hoʻohaʻahaʻa ʻia ka RNA hoʻomaʻemaʻe

ʻO ka maikaʻi o ka RNA i hoʻomaʻemaʻe ʻia e pili ana i nā mea e like me ka mālama ʻana i ka laʻana, RNase contamination, a me ka manipulation, etc.

1. ʻAʻole mālama ʻia nā ʻāpana kiko i ka manawa.

Manaʻo Manaʻo: Inā ʻaʻole hoʻohana ʻia nā laʻana a i ʻole nā ​​​​pūnaewele i ka manawa kūpono ma hope o ka hōʻiliʻili ʻana, e mālama koke i ka cryopreserve ma -80 °C a i ʻole ka wai nitrogen.No ka unuhi ʻana i ka RNA, e hoʻohana i kahi ʻiʻo i lawe ʻia a i ʻole ka laʻana cell i ka wā hiki.

2. ʻO ka hoʻoheheʻe hou ʻia ʻana o nā laʻana kiko.

Manaʻo Manaʻo: I ka wā e mālama ai i nā laʻana ʻiʻo, ʻoi aku ka maikaʻi e ʻoki ʻia i mau ʻāpana liʻiliʻi no ka mālama ʻana, a e wehe i kekahi o nā ʻāpana i ka wā e hoʻohana ai ia mau mea e pale aku ai i ka hoʻoheheʻe pinepine ʻana o ka hāpana a me ka hōʻino ʻana o RNA.

3. Hoʻokomo ʻia ʻo RNase a ʻaʻole hoʻohana i nā mīkina lima hoʻolele, masks, etc. i ka wā o ka hana.

Manaʻo Manaʻo: Hana maikaʻi ʻia nā hoʻokolohua RNA extraction ma nā keʻena hoʻoponopono RNA kaʻawale a holoi ʻia ka papaʻaina ma mua o ka hoʻokolohua.

E hoʻohana i nā mīkina lima a me nā maka i ka wā o ka hoʻokolohua e hōʻemi i ka hōʻemi ʻana o RNA ma muli o ka hoʻokomo ʻana o RNase.

4. Ho'ohaumia 'ia nā reagents me RNase i ka wā e ho'ohana ai.

Manaʻo Manaʻo: E hoʻololi me kahi Animal Total RNA Isolation Kit no nā hoʻokolohua pili.

5. ʻO nā paipu centrifuge, nā ʻōlelo aʻoaʻo, a me nā mea ʻē aʻe i hoʻohana ʻia i ka RNA manipulation ua haumia me RNase.

Manaʻo Manaʻo: E hōʻoia i ka RNase-Free nā paipu centrifuge, nā ʻōlelo aʻoaʻo, nā pipette, a me nā mea ʻē aʻe i hoʻohana ʻia i ka unuhi RNA.

Hoʻopili ka RNA i hoʻomaʻemaʻe ʻia i nā hoʻokolohua ma lalo

Hoʻomaʻemaʻe ʻia ʻo RNA e ke kolamu hoʻomaʻemaʻe, inā ʻoi aku ka nui o nā ion paʻakai, ʻoi aku ka nui o ka protein ma lalo o ka hoʻokolohua, e like me: reverse transcription, Northern Blot et al.

1. He koena iona paakai ko ka RNA i hooleiia.

Manaʻo Manaʻo: E hōʻoia ua hoʻohui ʻia ka nui kūpono o ka ethanol i ka Buffer RW2 a hana i 2 holoi kolamu hoʻomaʻemaʻe ma ka wikiwiki centrifugal i hōʻike ʻia no ka hana;inā loaʻa kekahi koena ion paʻakai, waiho i ke kolamu hoʻomaʻemaʻe iā Buffer RW2 no 5 min ma ka lumi wela a hana centrifugation e hoʻonui i ka wehe ʻana o ka paʻakai.

2. Ke koena ethanol i loko o ka RNA i hoʻoheheʻe ʻia.

Manaʻo Manaʻo: E hōʻoia ma hope o ka holoi ʻana i ka RW2, e hoʻokō i ka hana centrifugation paipu ʻole ma ka wikiwiki centrifugation i hōʻike ʻia no ka hana, e hoʻonui i ka manawa o ka hana centrifugation paipu ʻole i 2 min inā he koena ethanol, a i ʻole waiho ʻia ma ka lumi wela no 5 min ma hope o ka centrifugation pahu hakahaka e hoʻonui i ka wehe ʻana o ke koena ethanol.