Me ka hōʻaiʻē ʻoihana maikaʻi, ka lawelawe ma hope o ke kūʻai aku a me nā hale hana hou, ua loaʻa iā mākou kahi inoa maikaʻi loa i waena o kā mākou mea kūʻai aku ma ka honua holoʻokoʻa.hui piha o na reagents, Pono ka misionari o kā mākou paʻa e hāʻawi i nā waiwai kiʻekiʻe maikaʻi loa me ka uku kumukūʻai maikaʻi loa.Ua kakali mākou i ka hana ʻana me ʻoe!
Me ka hōʻaiʻē ʻoihana maikaʻi, ka lawelawe ma hope o ke kūʻai aku a me nā hale hana hou, ua loaʻa iā mākou kahi inoa maikaʻi loa i waena o kā mākou mea kūʻai aku ma ka honua holoʻokoʻa.hui piha o na reagents, Ma muli o nā waiwai me ke kūlana kiʻekiʻe, ke kumukūʻai hoʻokūkū, a me kā mākou lawelawe piha piha, i kēia manawa ua hōʻiliʻili mākou i ka ikaika kūpono a me kaʻike, a ua kūkulu mākou i kahi inoa maikaʻi loa ma ke kahua.Me ka hoʻomau mau ʻana, hāʻawi mākou iā mākou iho ʻaʻole wale i ka ʻoihana kūloko Kina akā i ka mākeke honua.E hoʻoneʻe ʻia ʻoe e kā mākou mau mea kiʻekiʻe a me ka lawelawe makemake.E wehe kākou i mokuna hou o ka pōmaikaʻi like a me ka lanakila pālua.

Nā kikoʻī

50 Mākaukau, 200 Mākaukau

Nā ʻāpana pahu

Nā hiʻohiʻona & pono

■ Hoʻohana ʻia ke kaʻina holoʻokoʻa ma ka lumi wela (15-25 ℃), me ka ʻauʻau hau a me ka centrifugation haʻahaʻa.
■ ʻO ka pahu holoʻokoʻa he RNase-Free, ʻaʻohe pono e hopohopo e pili ana i ka hōʻino ʻana o RNA.
■ Hoʻopaʻa pono ʻia ke kolamu hoʻomaʻemaʻe DNA i ka DNA, i hiki i ka pahu ke hoʻopau i ka ʻino DNA genomic me ka hoʻohui ʻole ʻana i ka DNase hou.
■ Loaʻa RNA kiʻekiʻe: Hiki i ke kolamu RNA-wale a me ka hoʻohālikelike kū hoʻokahi ke hoʻomaʻemaʻe pono i ka RNA.
■ ʻO ka wikiwiki wikiwiki: maʻalahi ka hana a hiki ke hoʻopau i nā minuke 11.
■ Palekana: ʻAʻole koi ʻia ka mea hoʻoponopono organik.
■ Ke kūlana kiʻekiʻe: ʻO ka RNA i hoʻomaʻemaʻe ʻia he maʻemaʻe kiʻekiʻe, ʻaʻohe protein a me nā mea haumia ʻē aʻe, a hiki ke hālāwai me nā hoʻokolohua like ʻole.

Ke noi pahu

He kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka nui o ka RNA mai nā cell moʻomeheu i nā papa 96, 24, 12, a me 6-well.

Ka holo hana

Diagram

Ua mālama ʻia ke kiʻi pākaukau agarose gel o ka Cell Total RNA Isolation Kit i nā helu like ʻole o luna, 20μl volume elution, e lawe i ka 2μl i hoʻomaʻemaʻe ʻia i ka RNA 1%.

Ka mālama ʻana a me ka mālama ʻana

Hiki ke mālama ʻia ka pahu no 12 mahina ma ka lumi wela (15–25 ℃) a me 2–8 ℃ no ka manawa lōʻihi (24 mau mahina).

Hiki ke mālama ʻia ʻo Buffer cRL1 ma 4 ℃ no ka mahina 1 ma hope o ka hoʻohui ʻana i ka 2-hydroxy-1-ethanethiol (koho).Ua kakali mākou i ka hana ʻana me ʻoe!
Factory pololei China Chemical Reagent, Research Chemical, Ma muli o nā waiwai me ke kūlana kiʻekiʻe, ke kumukūʻai hoʻokūkū, a me kā mākou lawelawe piha piha, i kēia manawa ua hōʻiliʻili mākou i ka ikaika kūpono a me ka ʻike, a ua kūkulu mākou i kahi inoa maikaʻi loa ma ke kahua.Me ka hoʻomau mau ʻana, hāʻawi mākou iā mākou iho ʻaʻole wale i ka ʻoihana kūloko Kina akā i ka mākeke honua.E hoʻoneʻe ʻia ʻoe e kā mākou mau mea kiʻekiʻe a me ka lawelawe makemake.E wehe kākou i mokuna hou o ka pōmaikaʻi like a me ka lanakila pālua.