ʻO kā mākou hoʻomaikaʻi ʻana e pili ana i nā lako ʻoi aku ka maikaʻi, nā talena maikaʻi loa a me nā pūʻali ʻenehana hoʻoikaika mau noe unuhi i ka RNA a pau, No ka ʻike hou aku e pili ana i ka mea hiki iā mākou ke hana iā ʻoe iho, e kamaʻilio me mākou i kēlā me kēia manawa.Manaʻo mākou i ka hoʻomohala ʻana i nā hui hui maikaʻi a lōʻihi me ʻoe.
ʻO kā mākou hoʻomaikaʻi ʻana e pili ana i nā lako ʻoi aku ka maikaʻi, nā talena maikaʻi loa a me nā pūʻali ʻenehana hoʻoikaika mau noe unuhi i ka RNA a pau, Ke hoʻohana nei ia i ka ʻōnaehana alakaʻi honua no ka hana hilinaʻi, kahi haʻahaʻa haʻahaʻa haʻahaʻa, kūpono ia no nā koho mea kūʻai aku ʻo Argentina.Aia kā mākou hui i loko o nā kūlanakauhale civili kūloko, kūpono loa ke kaʻa, kū hoʻokahi kūlana ʻāina a me nā kūlana waiwai.Ke ʻimi nei mākou i ka hana ʻana i ka poʻe, meticulous, brainstorm, kūkulu i ka manaʻo ʻoihana ʻoihana.ʻO ka hoʻokele maikaʻi koʻikoʻi, ka lawelawe kūpono, ke kumu kūʻai kūpono ma Argentina ʻo kā mākou kū ma ke kahua o ka hoʻokūkū.Inā pono, e ʻoluʻolu e hoʻokaʻaʻike mai iā mākou ma kā mākou pūnaewele a i ʻole kūkākūkā kelepona, e hauʻoli mākou e lawelawe iā ʻoe.

Nā kikoʻī

50 Mākaukau, 200 Mākaukau

Nā ʻāpana pahu

Nā hiʻohiʻona & pono

■ Hoʻohana ʻia ke kaʻina holoʻokoʻa ma ka lumi wela (15-25 ℃), me ka ʻauʻau hau a me ka centrifugation haʻahaʻa.
■ ʻO ka pahu holoʻokoʻa he RNase-Free, ʻaʻohe pono e hopohopo e pili ana i ka hōʻino ʻana o RNA.
■ Hoʻopaʻa pono ʻia ke kolamu hoʻomaʻemaʻe DNA i ka DNA, i hiki i ka pahu ke hoʻopau i ka ʻino DNA genomic me ka hoʻohui ʻole ʻana i ka DNase hou.
■ Loaʻa RNA kiʻekiʻe: Hiki i ke kolamu RNA-wale a me ka hoʻohālikelike kū hoʻokahi ke hoʻomaʻemaʻe pono i ka RNA.
■ ʻO ka wikiwiki wikiwiki: maʻalahi ka hana a hiki ke hoʻopau i nā minuke 11.
■ Palekana: ʻAʻole koi ʻia ka mea hoʻoponopono organik.
■ Ke kūlana kiʻekiʻe: ʻO ka RNA i hoʻomaʻemaʻe ʻia he maʻemaʻe kiʻekiʻe, ʻaʻohe protein a me nā mea haumia ʻē aʻe, a hiki ke hālāwai me nā hoʻokolohua like ʻole.

Ke noi pahu

He kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka nui o ka RNA mai nā cell moʻomeheu i nā papa 96, 24, 12, a me 6-well.

Ka holo hana

Diagram

Ua mālama ʻia ke kiʻi pākaukau agarose gel o ka Cell Total RNA Isolation Kit i nā helu like ʻole o luna, 20μl volume elution, e lawe i ka 2μl i hoʻomaʻemaʻe ʻia i ka RNA 1%.

Ka mālama ʻana a me ka mālama ʻana

Hiki ke mālama ʻia ka pahu no 12 mahina ma ka lumi wela (15–25 ℃) a me 2–8 ℃ no ka manawa lōʻihi (24 mau mahina).

Hiki ke mālama ʻia ka Buffer cRL1 ma 4 ℃ no 1 mahina ma hope o ka hoʻohui ʻana i ka 2-hydroxy-1-ethanethiol (koho). ʻO kā mākou hoʻomaikaʻi ʻana e hilinaʻi ʻia i nā mea ʻoi aku ka maikaʻi, nā talena maikaʻi loa a me ka hoʻoikaika ʻana i nā ʻenehana ikaika no ka hale hana ʻoihana no China Amino Acid L-Glutamine Raw Material for Healing Bone Skin and Muscle Tissue Dietary Supplement.Manaʻo mākou i ka hoʻomohala ʻana i nā hui hui maikaʻi a lōʻihi me ʻoe.
ʻOihana ʻOihana no Kina Amino Acid, Pharmaceutical, Ke hoʻohana nei ia i ka ʻōnaehana alakaʻi honua no ka hana hilinaʻi, kahi haʻahaʻa haʻahaʻa haʻahaʻa, kūpono ia i nā koho mea kūʻai aku ʻo Argentina.Aia kā mākou hui i loko o nā kūlanakauhale civili kūloko, kūpono loa ke kaʻa, kū hoʻokahi kūlana ʻāina a me nā kūlana waiwai.Ke ʻimi nei mākou i ka hana ʻana i ka poʻe, meticulous, brainstorm, kūkulu i ka manaʻo ʻoihana ʻoihana.ʻO ka hoʻokele maikaʻi koʻikoʻi, ka lawelawe kūpono, ke kumu kūʻai kūpono ma Argentina ʻo kā mākou kū ma ke kahua o ka hoʻokūkū.Inā pono, e ʻoluʻolu e hoʻokaʻaʻike mai iā mākou ma kā mākou pūnaewele a i ʻole kūkākūkā kelepona, e hauʻoli mākou e lawelawe iā ʻoe.