Kuhikuhi RT-qPCR Kit
ʻO ka wehewehe ʻana o ka pahu:
◮ Direct RT-qPCR Kit, me ka hoʻohana ʻana i ka Foreasy Reverse Transcriptase a me ka Foreasy HS Taq DNA Polymerase e hoʻomohala ʻia e Foregene, me ka pale kūʻokoʻa kūʻokoʻa, hana i kēia kit me ke kūpaʻa ikaika a me ka hoʻohālikelike ʻana, a hiki iā ia ke hoʻāʻo pololei i ka hoʻohana ʻana i ka ʻōnaehana Foregene Lysis e like me ka laʻana.Hiki ke hoʻohana ʻia kēia pahu no ka pahu ʻike ʻike nucleic acid COVID-19 a me nā mea ʻē aʻe pathogenic bacteria nucleic acid detection kit development.
wehewehe
Hāʻawi ʻo Direct RT-qPCR Kit i hoʻopaʻa ʻokoʻa ʻia ʻo RT-qPCR buffer a me ka enzyme kiʻekiʻehui ʻia (Taq, M-MLV, RI), maʻalahi no ka hana ʻana i nā pahu hope a me nā ʻōnaehana.hoʻoponopono, ma ka hōʻoia maʻalahi.
Nā kikoʻī
500T, 50,000T
Nā ʻāpana pahu
| Kiko maʻiʻo (20 μL ʻōnaehana pane) | IM-05111-01 | IM-05112-01 |
| 500 T | 50,000 T | |
| Huihui Enzyme pololei | 500 μL×1 | 50 mL×1 |
| 2× Kuhi pololei RT-qPCR Buffer | 1 mL×5 | 500 mL×1 |
| Aʻo | 1 | 1 |
Nā ʻanuʻu hana
Ma mua o ka hoʻomākaukau ʻana i ka reagent, pono e hoʻoheheʻe ʻia nā reagents a pau i loko o ka pahu i kahi mahana wela a hui mālie.
A: Hoʻomākaukau of laʻana a reagent
1. E hoʻomākaukau i ka laʻana RNA i hoʻomākaukau ʻia (Aia ʻia e hoʻohana i ka Foregene Total RNA Isolation Kit series kit e unuhi a hoʻomaʻemaʻe i ka RNA template) a i ʻole nā huahana hoʻoheheʻe ʻia (Ua paipai ʻia e hoʻohana i ka ʻōnaehana Foregene Lysis), nā primers kikoʻī (10 μM) a me nā mea hoʻohana pono ʻē aʻe.
2. E kau i ka 2× Direct RT-qPCR Buffer, RNase-Free ddH2O a me 20× ROX Reference Dye (inā he's pono) i loko o ka pahu me ka hau, e hoohehee maoli ai keia mau mea, a hui malie.
B: Hoʻomākaukau of RT-qPCR ʻōnaehana
Papakaukau 1 : Hoʻomākaukau of RT-qPCR system
| ʻāpana | Volume | Hoʻoholo hope |
| 2× Kuhi pololei RT-qPCR Buffer | 10 μL | 1× |
| Huihui Enzyme pololei | 1μL | |
| Kuhi mua (10 μM) | 0.8 μL | 50-900nM |
| Pumi Hoʻohuli (10 μM) | 0.8 μL | 50-900nM |
| ʻImi (4 μM) | 1μL | 200nM |
| Palapala (RNA a i ʻole Lysate) | X μL | |
| 20×ROX Kuhikuhi Wailu | - | 1* |
| RNase-FreeddH2O | (6.4-X) μL | |
| Huina Volume | 20μL |
C: Hoʻonoho ka RT-qPCR pane ʻana papahana
Papakaukau 2 : RT-qPCR reaction papahana hoʻonohonoho (no ka mea laʻana: ʻelua-ʻanuʻu ʻano hana)
| ʻanuʻu | Mahana | Manawa | Nā pōʻaiapuni | Maʻiʻo | |
| 1 | 50 ℃ | 15 min | 1* | 1 | Hoʻihoʻi i ka palapala |
| 2 | 95 ℃ | 1 min | 1 | Pre-denaturation | |
| 3 | 95 ℃ | 10 sec | 40-45 | Denaturation | |
| 60 ℃ | 30 kekona 2* | Anneal/Hoʻonui | |||
Ka mālama ʻana a me ka mālama ʻana
- Pono e mālama ʻia ka pahu ma -20± 5℃.ʻO 12 mahina ka manawa kūpono.
-E hōʻalo i nā pōʻai maloʻo maloʻo (<5 mau pōʻai).
