Hiki iā mākou ke hōʻoluʻolu mau i kā mākou mea kūʻai aku i mahalo ʻia me kā mākou kūlana kiʻekiʻe maikaʻi, uku kumukūʻai maikaʻi a me ke kākoʻo maikaʻi no ka mea ua lilo mākou i mea loea hou a ʻoi aku ka paʻakikī a hana i ke ʻano kumu kūʻai no ka maikaʻi maikaʻi Kina Highly Efficient and Stable Lyophilized Dspathtm 4X One-Step Multiplex Master Mix, E hōʻoia ʻoe i ka manaʻo ʻole loa e kamaʻilio me mākou no ka hui.a ke manaʻoʻiʻo nei mākou e kaʻana like i ka ʻike kūpono kālepa kūpono me kā mākou poʻe kālepa āpau.
Hiki iā mākou ke hōʻoluʻolu mau i kā mākou mea kūʻai aku i mahalo ʻia me kā mākou kiʻekiʻe kiʻekiʻe, ke kumu kūʻai maikaʻi a me ke kākoʻo maikaʻi no ka mea ua lilo mākou i mea loea a ʻoi aku ka paʻakikī a hana i ke ala kūpono no kaKina Taq DNA Polymerase, Qpcr, Ke hoʻomau nei kā mākou hui i nā mea kūʻai aku me ke kūlana kiʻekiʻe, ke kumukūʻai hoʻokūkū a me ka hāʻawi manawa.Hoʻokipa maikaʻi mākou i nā hoaaloha mai ka honua holoʻokoʻa e hui pū me mākou a hoʻonui i kā mākou ʻoihana.Inā makemake ʻoe i kā mākou huahana, pono ʻoe e hoʻokaʻaʻike mai iā mākou.Makemake mākou e hāʻawi iā ʻoe me ka ʻike hou aku.

Nā kikoʻī

5ml, 10ml, 40ml

ʻO ka pahu PCR Hero (Me ka Dye) Extraction kit e pili ana i ka hanauna hou o Foregene o Taq—Foregene Taq Hero, nā dNTP i hoʻopaʻa ʻia, MgCl2, hoʻopaʻa hopena, PCR reaction enhancer, optimizer a me stabilizer.ʻO ka ʻōnaehana 2× PCR HeroTM Mix (dye) ʻoi aku ka kiʻekiʻe o ka hoʻomanawanui ʻana i ka PCR inhibitors ma mua o ka ʻōnaehana PCR Mix maʻamau, a hiki ke maʻalahi i ka PCR amplification o nā ʻano paʻakikī like ʻole.ʻO ka ʻōnaehana hoʻololi kū hoʻokahi a me ka Taq Hero kiʻekiʻe e hana i ka hopena PCR i ʻoi aku ka kiʻekiʻe o ka amplification pono, kikoʻī a me ka naʻau.

Nā ʻāpana pahu

Nā hiʻohiʻona & pono

■ ʻOi aku ka hilinaʻi: 6 manawa i ko Taq enzyme maʻamau;

■ ʻOi aku ka wikiwiki o ka hoʻonui ʻana : ʻo 5-10sec/kb ka māmā hoʻonui, ʻo ia ka 6-12 mau manawa i ko Taq enzyme maʻamau;

■ Hiki ke hoʻololi hou aku i ka laʻana: Hiki iā ia ke hoʻonui i nā ʻano maʻamau DNA paʻakikī me ke kiʻekiʻeGC maʻiʻo;

■ ʻOi aku ka maikaʻi o ka hoʻonui ʻana: nā pōʻai hoʻonui haʻahaʻa ma mua o ka enzyme Taq maʻamau;

■ ʻOi aku ka ikaika o ka hoʻomanawanui ʻana o ke kaiapuni: kau ʻia ma 37°C no hoʻokahi pule, mālama ʻia ma mua o 90% hana;

■ He 5′ →3′ DNA polymerase hana a me 5′→3′ exonuclease hana, me ka 3′ →5′ hana exonuclease.

■ Ua hui mua ka pahu me ka Electrophoresis Loading Buffer, ma hope o ka pau ʻana o ka PCR, hiki ke hoʻouka pololei ʻia ka hāpana no ka electrophoresis;maʻalahi a maʻalahi ka hana.

Ke noi pahu

Hoʻonui ʻia ka PCR o nā ʻāpana DNA, ka hōʻailona DNA, ka hoʻopili ʻana i ka DNA, PCR me kahi huelo

Ka mālama ʻana a me ka mālama ʻana

Hiki ke mālama ʻia no ka manawa lōʻihi ma -20 ° C;no ka hoʻohana pinepine ʻana, hiki ke mālama ʻia ma 4 ° C no kahi manawa pōkole. Hiki iā mākou ke hoʻomāʻona mau i kā mākou mea kūʻai aku me ko mākou kūlana kiʻekiʻe kiʻekiʻe, ke kumu kūʻai maikaʻi a me ke kākoʻo maikaʻi no ka mea ua lilo mākou i loea hou a ʻoi aku ka paʻakikī a hana ia ma ke ʻano kumu kūʻai no ka maikaʻi maikaʻi Kina Highly Efficient and Stable Lyophilized Dspathtm ʻO ka hui ʻana i ka manaʻo nui ʻole iā ʻoe e ʻōlelo iā 4X Masterely One-Step.a ke manaʻoʻiʻo nei mākou e kaʻana like i ka ʻike kūpono kālepa kūpono me kā mākou poʻe kālepa āpau.
ʻAno maikaʻi loaKina Taq DNA Polymerase, Qpcr, Ke hoʻomau nei kā mākou hui i nā mea kūʻai aku me ke kūlana kiʻekiʻe, ke kumukūʻai hoʻokūkū a me ka hāʻawi manawa.Hoʻokipa maikaʻi mākou i nā hoaaloha mai ka honua holoʻokoʻa e hui pū me mākou a hoʻonui i kā mākou ʻoihana.Inā makemake ʻoe i kā mākou huahana, pono ʻoe e hoʻokaʻaʻike mai iā mākou.Makemake mākou e hāʻawi iā ʻoe me ka ʻike hou aku.