Manaʻo nui ʻia kā mākou huahana a hilinaʻi ʻia e nā mea kūʻai aku a hiki ke hoʻokō mau i nā koi kālā a me ka pilikanaka no Factory pololei Hipure Rna Column Viral Rna Column, Mai ka wā i hoʻokumu ʻia ai ka hale hana, i kēia manawa ua hana mākou i ka holomua o nā mea kūʻai hou.Me ka wikiwiki o ka nohona a me ka hoʻokele waiwai, e hoʻomau mākou i ka lawe ʻana i ka ʻuhane o "kiʻekiʻe kiʻekiʻe kiʻekiʻe, kūpono, hana hou, kūpaʻa", a hahai i ke kumu hana o "aie mua, mea kūʻai mua, maikaʻi maikaʻi maikaʻi".E hana mākou i kahi holo lōʻihi i ka hana lauoho me kā mākou mau hoa.
Manaʻo nui ʻia kā mākou huahana a hilinaʻi ʻia e nā mea kūʻai aku a hiki ke hoʻokō mau i nā koi kālā a me ka pilikanakaʻO China Spin Silica Membrane Column a me DNA/Rna Extraction Purification Isolation, No ka nui o ka 'oihana.e nā hoa, ua hoʻonui mākou i ka papa inoa o nā mea a ʻimi i ka laulima maikaʻi.Hōʻike kā mākou pūnaewele i ka ʻike a me ka ʻike piha e pili ana i kā mākou papa inoa kūʻai a me ka hui.No ka ʻike hou aku, e pane koke kā mākou hui lawelawe kūkākūkā ma Bulgaria i nā nīnau āpau a me nā pilikia.Ke manaʻo nei lākou e hana i kā lākou hana maikaʻi loa e hālāwai me nā mea kūʻai aku e pono ai.Kākoʻo nō hoʻi mākou i ka hāʻawi ʻana i nā laʻana manuahi loa.Hoʻokipa ʻia nā kipa ʻoihana i kā mākou ʻoihana ma Bulgaria a me ka hale hana no ke kūkākūkā win-win.Manaʻolana e ʻike i kahi hui hauʻoli e hana pū me ʻoe.

Nā wehewehe kime

Hiki ke unuhi ʻia a hoʻomaʻemaʻe ʻia nā huahana nucleic acid o FOREGENE mai nā kumu like ʻole a me nā mea hoʻohālike paʻakikī.Hoʻokumu ʻia nā huahana DNA extraction series ma nā kolamu hoʻomaʻemaʻe ʻana o ka membrane silica DNA wale nō a me nā reagents formula kūikawā hiki ke hoʻomaʻemaʻe i ka DNA kiʻekiʻe mai nā ʻano laʻana.

Hoʻohana ka General Plasmid Mini Kit i ka ʻenehana kolamu hoʻomaʻemaʻe DNA-Only a me kahi formula lysis SDS kūpono e loaʻa ai ka DNA plasmid kiʻekiʻe mai ka bacteria i loko o 20 mau minuke.ʻO ka DNA-Only silica gel membrane i hoʻohana ʻia i loko o ke kolamu spin he mea kūʻokoʻa hou ia o FOREGENE, hiki iā ia ke hoʻopili i ka DNA me ka maikaʻi a kikoʻī, a hoʻoneʻe i ka RNA, nā protein haumia, nā ion a me nā mea hoʻohui ʻē aʻe i loko o nā cell i ka nui loa.ʻO ka mana hoʻopaʻa paʻa kiʻekiʻe o ka DNA plasmid o ka kolamu hoʻomaʻemaʻe DNA-Only he 60μg DNA.ʻO ka nui o ka DNA plasmid i unuhi maoliʻia e pili ana i ka nui o ka hopena bacteria, ka helu o nā kope plasmid (kope kiʻekiʻe, kope haʻahaʻa), nā kānana, nā kūlana moʻomeheu a me nā mea'ē aʻe.

Nā kikoʻī

Nā hiʻohiʻona & pono

- ʻAʻohe RNase contamination: ʻO ka DNA-Only Column i hāʻawi ʻia e ka kit e hiki ai ke wehe i ka RNA i loko o ka DNA plasmid me ka hoʻohui ʻole ʻana i ka RNase i ka wā o ka hoʻokolohua, e pale ana i ka hoʻopōʻino ʻia o ka hale hana e ka exogenous RNase.

- Ka wikiwiki wikiwiki: Hiki ke hoʻopau i ka unuhi ʻana o Plasmid DNA i loko o 20 mau minuke.

- Palekana: ʻAʻole koi ʻia ka unuhi ʻana o ka reagent.

- Kiʻekiʻe kiʻekiʻe: ʻO ka plasmid i loaʻa ma ka unuhi ʻana ʻaʻohe RNA, ʻaʻohe RNase a me ka haʻahaʻa haʻahaʻa haʻahaʻa, hiki ke hoʻokō i nā koi o nā hoʻokolohua like ʻole.

Ke noi pahu

ʻO ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka DNA Plasmid no ka maʻi bacteria Gram-negative.

Ka holo hana

Diagram

Ka mālama ʻana a me ka mālama ʻana

Hiki ke mālama ʻia kēia kit no 12 mau mahina ma lalo o nā kūlana maloʻo ma ke ana wela (15-25 ℃);inā pono e mālama ʻia no ka manawa lōʻihi, hiki ke mālama ʻia ma 2-8 ℃.

'Ōlelo Aʻo: Inā mālama ʻia ma kahi wela haʻahaʻa, hiki i ka hāʻina ke hiki i ka ua.Ma mua o ka hoʻohana ʻana, e hōʻoia e waiho i ka hopena i loko o ka pahu i ka lumi wela no kekahi manawa.Inā pono, e hoʻomaʻamaʻa mua iā ia i loko o kahi ʻauʻau wai 37 ℃ no 10 mau minuke e hoʻoheheʻe i ka wai, a hui pū ma mua o ka hoʻohana ʻana.

Manaʻo nui ʻia kā mākou huahana a hilinaʻi ʻia e nā mea kūʻai aku a hiki ke hoʻokō mau i nā koi kālā a me ka pilikanaka no Factory pololei Hipure Rna Column Viral Rna Column, Mai ka wā i hoʻokumu ʻia ai ka hale hana, i kēia manawa ua hana mākou i ka holomua o nā mea kūʻai hou.Me ka wikiwiki o ka nohona a me ka hoʻokele waiwai, e hoʻomau mākou i ka lawe ʻana i ka ʻuhane o "kiʻekiʻe kiʻekiʻe kiʻekiʻe, kūpono, hana hou, kūpaʻa", a hahai i ke kumu hana o "aie mua, mea kūʻai mua, maikaʻi maikaʻi maikaʻi".E hana mākou i kahi holo lōʻihi i ka hana lauoho me kā mākou mau hoa.
Hale hana pololeiʻO China Spin Silica Membrane Column a me DNA/Rna Extraction Purification Isolation, No ka nui o ka 'oihana.e nā hoa, ua hoʻonui mākou i ka papa inoa o nā mea a ʻimi i ka laulima maikaʻi.Hōʻike kā mākou pūnaewele i ka ʻike a me ka ʻike piha e pili ana i kā mākou papa inoa kūʻai a me ka hui.No ka ʻike hou aku, e pane koke kā mākou hui lawelawe kūkākūkā ma Bulgaria i nā nīnau āpau a me nā pilikia.Ke manaʻo nei lākou e hana i kā lākou hana maikaʻi loa e hālāwai me nā mea kūʻai aku e pono ai.Kākoʻo nō hoʻi mākou i ka hāʻawi ʻana i nā laʻana manuahi loa.Hoʻokipa ʻia nā kipa ʻoihana i kā mākou ʻoihana ma Bulgaria a me ka hale hana no ke kūkākūkā win-win.Manaʻolana e ʻike i kahi hui hauʻoli e hana pū me ʻoe.