ʻO ka ʻoluʻolu o ka mea kūʻai aku ko mākou manaʻo nui.Ke kākoʻo nei mākou i ka pae kūpono o ka ʻoihana, ka maikaʻi kiʻekiʻe, ka hilinaʻi a me ka lawelawe no Hipure PCR Pure Mini Kit Recover DNA Fragments Ma waena o 60bp-20kbp, Welina mai i kāu mākaʻikaʻi a me kāu mau nīnau, manaʻolana nui e hiki iā mākou ke loaʻa ka manawa e hui pū me ʻoe a e kūkulu mākou i ka male ʻoihana nui me ʻoe.
ʻO ka ʻoluʻolu o ka mea kūʻai aku ko mākou manaʻo nui.Kūpaʻa mākou i kahi pae kūlike o ka ʻoihana, ʻoi aku ka maikaʻi, ka hilinaʻi a me ka lawelawe noʻO Kina Rna/DNA Hoʻomaʻemaʻe Hoʻomaʻemaʻe a me Nucleic Acid Isolation, Ua hoʻokumu mākou i nā pilina ʻoihana lōʻihi, kūpaʻa a maikaʻi me nā mea hana a me nā mea kūʻai nui a puni ka honua.I kēia manawa, ke kakali nei mākou i ka launa pū ʻana me nā mea kūʻai aku ma waho e pili ana i nā pono like.E ʻoluʻolu e hoʻokaʻaʻike mai iā mākou no nā kikoʻī hou aku.

Nā kikoʻī

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Nā ʻāpana pahu

Nā hiʻohiʻona & pono

■ ʻAʻohe hoʻomaʻemaʻe DNA hoʻopau manawa a pipiʻi

■ He liʻiliʻi ka noi hāpana, hiki ke hoʻāʻo ʻia ka ʻiʻo holoholona 5mg.

■ ʻAʻole koi ʻia kahi hana kūikawā e like me ka wili a me ka ʻoki ʻana, a maʻalahi ka hana.

■ ʻOi aku ka maikaʻi o ka PCR ʻoi aku ka maikaʻi o ka hoʻomanawanui ʻana i ka PCR reaction inhibitors.

Nā palena kime

Hoʻohana: nā ʻano ʻiʻo holoholona.

Hoʻokuʻu ʻia ka DNA mai ka laʻana lysis: hoʻohana wale ʻia ma ke ʻano he PCR template.

Hiki ke hoʻohana ʻia ke kit no nā kumu penei: ʻike i ka transgenosis, genotyping holoholona, ​​etc.

Ka holo hana

Ka mālama ʻana a me ka mālama ʻana

Pono e mālama ʻia ka ʻāpana I o kēia pahu ma 2-8 ℃.

Hiki ke mālama ʻia ka reagent Buffer AL no 12 mahina ma lalo o nā kūlana maloʻo;hiki ke mālama ʻia ma 2-8 ℃ no ka mālama lōʻihi.

Loaʻa i ka Reagent Foregene Protease kahi ʻano kūʻokoʻa.No ka hōʻoia ʻana i kāna hana a paʻa, e ʻoluʻolu e mālama iā ia ma 4 ℃ no 12 mahina.

Hiki ke mālama ʻia ka Reagent 6×DNA Loading Buffer ma 4 ℃ a i ʻole -20 ℃ no ka manawa lōʻihi.

Pono e mālama ʻia ka ʻāpana II o kēia pahu ma -20 ℃.

Reagent 2×PCR maʻalahi^TMHiki ke mālama ʻia ka hui ʻana no 12 mau mahina ma -20 ℃;inā hoʻohana pinepine ʻia, hiki ke mālama ʻia ma 4 ℃ no ka mālama pōkole (hoʻohana i loko o 10 mau lā).

ʻO ka ʻoluʻolu o ka mea kūʻai aku ko mākou manaʻo nui.Ke kākoʻo nei mākou i ka pae kūpono o ka ʻoihana, ka maikaʻi kiʻekiʻe, ka hilinaʻi a me ka lawelawe no Hipure PCR Pure Mini Kit Recover DNA Fragments Ma waena o 60bp-20kbp, Welina mai i kāu mākaʻikaʻi a me kāu mau nīnau, manaʻolana nui e hiki iā mākou ke loaʻa ka manawa e hui pū me ʻoe a e kūkulu mākou i ka male ʻoihana nui me ʻoe.
Hoʻolālā maikaʻi ʻiaʻO Kina Rna/DNA Hoʻomaʻemaʻe Hoʻomaʻemaʻe a me Nucleic Acid Isolation, Ua hoʻokumu mākou i nā pilina ʻoihana lōʻihi, kūpaʻa a maikaʻi me nā mea hana a me nā mea kūʻai nui a puni ka honua.I kēia manawa, ke kakali nei mākou i ka launa pū ʻana me nā mea kūʻai aku ma waho e pili ana i nā pono like.E ʻoluʻolu e hoʻokaʻaʻike mai iā mākou no nā kikoʻī hou aku.