Hoʻoikaika pū mākou i ka hoʻoikaika ʻana i nā mea hoʻokele a me ke ʻano QC e hōʻoia i hiki iā mākou ke mālama i ka waiwai nui ʻoiai i ka ʻoihana hoʻokūkū ikaika no Factory For China Techstar Magnetic Bead Method Swab Sample Nucleic Acid Extraction Kit Rna Isolation Reagent DNA Purification Kit, ʻike manaʻoʻiʻo!Hoʻokipa maikaʻi mākou i nā manaʻo hou ma waho e hoʻonohonoho i nā pilina ʻoihana a manaʻo pū kekahi e hoʻohui i nā pilina me nā mea kūʻai aku lōʻihi.
Hoʻoikaika pū mākou i ka hoʻoikaika ʻana i nā mea hoʻokele a me ke ʻano QC e hōʻoia i hiki iā mākou ke mālama i ka waiwai nui ʻoiai i ka ʻoihana hoʻokūkū ikaika.Kina Nucleic Acid Reagent, ʻO ka pahu hoʻoheheʻe DNA/Rna, He mea hoʻolako honua kā mākou hui ma kēia ʻano mea kūʻai.Hāʻawi mākou i kahi koho kupaianaha o nā hāʻina kiʻekiʻe.ʻO kā mākou pahuhopu ka leʻaleʻa iā ʻoe me kā mākou hōʻiliʻili kūʻokoʻa o nā mea noʻonoʻo ʻoiai e hāʻawi ana i ka waiwai a me ka lawelawe maikaʻi loa.He maʻalahi kā mākou misionari: E hāʻawi i nā huahana a me nā lawelawe maikaʻi loa i kā mākou mea kūʻai aku ma nā kumukūʻai haʻahaʻa loa.

Nā kikoʻī

50 Preps, no ka hoʻomaʻemaʻe DNA genomic mai ke koko holoʻokoʻa e hoʻohana ana<1mlkoko

Buffer DC: Hoʻokuʻu i nā ʻāpana koko ʻulaʻula i loko o ke koko.

Buffer BL1: Hāʻawi i kahi laʻana kaiapuni hoʻoheheʻe enzymatic.

Foregene Protease Plus: Enzymatically digest koko laʻana i loko o ke kaiapuni o Buffer BL1.

Buffer BL2: Hoʻopau i ka Foregene Protease Plus a hāʻawi i kahi wahi hoʻouka DNA.

Buffer PW: Wehe i nā haumia e like me ka protein a me ka RNA ma DNA.

Buffer WB1: Wehe i nā koena paʻakai ma DNA.

Buffer EB: Hoʻopili i ka DNA ma ka membrane kolamu hoʻomaʻemaʻe.

DNA-Wae wale: Hoʻopili pono i ka DNA genomic i ka lysate.

Nā ʻāpana pahu

Nā hiʻohiʻona & pono

-ʻAʻohe RNase contamination: ʻO ka DNA-Only Column i hāʻawi ʻia e ka kit e hiki ai ke wehe i ka RNA mai ka genomic DNA me ka ʻole o ka hoʻohui ʻana i ka RNase i ka wā o ka hoʻokolohua, e pale ana i ka hale hana mai ka haumia ʻana e ka exogenous RNase.

ʻO ka wikiwiki wikiwiki: ʻoi aku ka ʻoi aku o ka ʻoihana Foregene Protease Plus ma mua o nā protease like, a ʻoi aku ka wikiwiki o ka hoʻoemi ʻana i nā ʻāpana kiko;maʻalahi ka hana, a hiki ke hoʻopau ʻia ka hana unuhi DNA genomic i loko o 40 mau minuke.

-ʻoluʻolu: Hana ʻia ka centrifugation ma ka lumi wela, 4 ° C centrifugation a i ʻole ethanol precipitation o DNA ʻaʻole pono.

-Palekana: ʻAʻole hoʻohana ʻia kahi hoʻoheheʻe reagent organik.

-Kiʻekiʻe kiʻekiʻe: ʻO nā ʻāpana DNA genomic i unuhi ʻia he nui, ʻaʻohe RNA, ʻaʻohe RNase, a me ka haʻahaʻa haʻahaʻa haʻahaʻa, hiki ke hoʻokō i nā koi o nā hoʻokolohua like ʻole.

Ke noi pahu

He kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka DNA genomic mai ke koko holoʻokoʻa anticoagulated hou a maloʻo paha.

Kaʻina hana

Ka mālama ʻana a me ka mālama ʻana

Hiki ke mālama ʻia kēia kime no 12 mau mahina ma lalo o nā kūlana maloʻo ma ke ana wela (15-25 ° C);inā pono e mālama ʻia no ka manawa lōʻihi, hiki ke mālama ʻia ma 2-8 ° C.

'Ōlelo Aʻo: Inā mālama ʻia ma kahi wela haʻahaʻa, hiki i ka hāʻina ke hiki i ka ua.Ma mua o ka hoʻohana ʻana, e hōʻoia e waiho i ka hopena i loko o ka pahu i ka lumi wela no kekahi manawa.Inā pono, e hoʻomaʻamaʻa i ka wai ʻauʻau 37 ° C no 10 mau minuke e hoʻoheheʻe i ka wai, a hui ʻia ma mua o ka hoʻohana ʻana.

ʻO ka hoʻonā Foregene Protease Plus he kumu kūʻokoʻa, e hana ana i ka wā e mālama ʻia i ka lumi lumi no ka manawa lōʻihi (3 mau mahina);e ʻoi aku ka ikaika a paʻa ke mālama ʻia ma 4 ° C, no laila e ʻōlelo ʻia e mālama iā ia ma 4 ° C, e hoʻomanaʻo ʻaʻole e mālama iā ia ma -20 ° C. Hoʻoikaika pū mākou i ka hoʻoikaika ʻana i nā mea hoʻokele a me ke ʻano QC e hōʻoia ai e hiki iā mākou ke mālama i ka waiwai nui ma ka ʻoihana hoʻokūkū ikaika no ka Factory For China Techstar Magnetic Bead Method E ʻike i ka Acid Sample Nucle Kit!Hoʻokipa maikaʻi mākou i nā manaʻo hou ma waho e hoʻonohonoho i nā pilina ʻoihana a manaʻo pū kekahi e hoʻohui i nā pilina me nā mea kūʻai aku lōʻihi.
Hale Hana NoKina Nucleic Acid Reagent, ʻO ka pahu hoʻoheheʻe DNA/Rna, He mea hoʻolako honua kā mākou hui ma kēia ʻano mea kūʻai.Hāʻawi mākou i kahi koho kupaianaha o nā hāʻina kiʻekiʻe.ʻO kā mākou pahuhopu ka leʻaleʻa iā ʻoe me kā mākou hōʻiliʻili kūʻokoʻa o nā mea noʻonoʻo ʻoiai e hāʻawi ana i ka waiwai a me ka lawelawe maikaʻi loa.He maʻalahi kā mākou misionari: E hāʻawi i nā huahana a me nā lawelawe maikaʻi loa i kā mākou mea kūʻai aku ma nā kumukūʻai haʻahaʻa loa.