Hoʻolālā maikaʻi ʻia ʻo Kina PCR ʻO Sybr Green Qpcr Mix (Kiʻekiʻe ROX+)
ʻO ka wehewehe ʻana o ka pahu:
ʻO ka ʻōnaehana pane kū hoʻokahi a me ka Taq DNA Polymerase kiʻekiʻe e hana i ka hopena PCR i ʻoi aku ka kiʻekiʻe o ka amplification pono, kikoʻī a me ka naʻau.
No ka hana ʻana i nā kumukūʻai ʻoi aku ka nui o nā mea kūʻai aku ʻo kā mākou ʻoihana ʻoihana;ʻO ka mea kūʻai e ulu nei kā mākou hana hana no Kina i hoʻolālā maikaʻi ʻia ʻo PCR Sybr GreenQpcrHoʻohui (High ROX +), No ka loaʻa ʻana o kā mākou mana OEM / ODM ikaika a me nā huahana a me nā lawelawe noʻonoʻo, e ʻoluʻolu e kelepona iā mākou i kēia lā.E hoʻomohala ʻoiaʻiʻo mākou a e kaʻana like i ka holomua me nā mea kūʻai aku a pau.
No ka hana ʻana i nā kumukūʻai ʻoi aku ka nui o nā mea kūʻai aku ʻo kā mākou ʻoihana ʻoihana;ʻO ka ulu ʻana o ka mea kūʻai aku ʻo kā mākou hana hanaKina Taq DNA Polymerase, Qpcr, No ka poʻe makemake i kekahi o kā mākou waiwai ma hope o kou nānā ʻana i kā mākou papa inoa huahana, e ʻoluʻolu e ʻoluʻolu e hoʻokaʻaʻike mai iā mākou no nā nīnau.Hiki iā ʻoe ke hoʻouna mai iā mākou i nā leka uila a hoʻokaʻaʻike mai iā mākou no ke kūkākūkā a pane mākou iā ʻoe i ka wā hiki iā mākou.Inā maʻalahi, hiki iā ʻoe ke ʻimi i kā mākou helu wahi ma kā mākou pūnaewele a hele mai i kā mākou ʻoihana no ka ʻike hou aku o kā mākou hoʻonā ʻana iā ʻoe iho.Mākaukau mau mākou e kūkulu i nā pilina pili lōʻihi a paʻa me nā mea kūʻai aku ma nā kahua pili.
Nā kikoʻī
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Nā ʻāpana pahu
| Mahele I | Palena P1 |
| Palena P2 | |
| 6×DNA Loading Buffer | |
| Mahele II | 2× Lau PCR maʻalahiTMHui (UNG) |
Nā hiʻohiʻona & pono
■ ʻAʻohe hoʻomaʻemaʻe DNA hoʻopau manawa a pipiʻi
■ He mea uuku
■ Maʻalahi-hiki ke hāʻawi ʻia ka laʻana i ka PCR a i ʻole ka hopena lysis me ka ʻoki ʻole a wili ʻole
■ 2 × Hoʻohui, hōʻemi i ka hāpana hoʻouka hewa a me ka pane ʻōnaehana manawa hoʻomākaukau
■ Hiki ke hoʻomākaukau i ka hoʻomākaukau wikiwiki i 10 mau minuke, hiki ke hoʻopau i ka hopena PCR i loko o 50 mau minuke.
■ Hiki ke hoʻopau ʻia ka hopena o ka throughput-lysis kiʻekiʻe ma ka pā 96-well PCR
Nā palena kime
Hoʻohana: genetically modified identification, genotyping, etc.
Laʻana: lau mea kanu
Ka hoʻopaʻa ʻana: ʻO ke anawaena 2-3mm (ke ala pololei), ke anawaena 5-7mm (ke ʻano lysis)
ʻIke ʻike: ʻāpana ʻāpana ≤1kb
Ka holo hana
Electtropherogram
ʻElua mau pule o ka ʻike ʻana i nā hopena PCR Mix ma lalo o ke ʻano o ka PCR amplification product contamination.ʻO ka mea kūʻai aku e ulu ana kā mākou hana hana no ka China Real Time PCR Sybr Green Qpcr Mix (High ROX +), No ka loaʻa ʻana o kā mākou mana OEM / ODM ikaika a me nā huahana a me nā lawelawe noʻonoʻo, e ʻoluʻolu e hoʻopili iā mākou i kēia lā.E hoʻomohala ʻoiaʻiʻo mākou a e kaʻana like i ka holomua me nā mea kūʻai aku a pau.
Hoʻolālā maikaʻi ʻiaKina Taq DNA Polymerase, Qpcr, No ka mea makemake i kekahi o kā mākou waiwai ma hope pono o kou ʻike ʻana i kā mākou papa inoa huahana, e ʻoluʻolu e ʻoluʻolu e hoʻokaʻaʻike mai iā mākou no nā nīnau.Hiki iā ʻoe ke hoʻouna mai iā mākou i nā leka uila a hoʻokaʻaʻike mai iā mākou no ke kūkākūkā a pane mākou iā ʻoe i ka wā hiki iā mākou.Inā maʻalahi, hiki iā ʻoe ke ʻimi i kā mākou helu wahi ma kā mākou pūnaewele a hele mai i kā mākou ʻoihana no ka ʻike hou aku o kā mākou hoʻonā ʻana iā ʻoe iho.Mākaukau mau mākou e kūkulu i nā pilina pili lōʻihi a paʻa me nā mea kūʻai aku ma nā kahua pili.
