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Laʻana manuahi no Kina High Sensitivity DNA/Rna One-Step Probe Rt-Qpcr Kit V2

Hoʻohālike manuahi no Kina High Sensitivity DNA/Rna One-Step Probe Rt-Qpcr Kit V2 Kiʻi Hōʻikeʻike
  • Laʻana manuahi no Kina High Sensitivity DNA/Rna One-Step Probe Rt-Qpcr Kit V2
  • Laʻana manuahi no Kina High Sensitivity DNA/Rna One-Step Probe Rt-Qpcr Kit V2
  • Laʻana manuahi no Kina High Sensitivity DNA/Rna One-Step Probe Rt-Qpcr Kit V2

ʻO ka wehewehe ʻana o ka pahu:

◮Hoʻokahi-ʻanuʻu pahu hana hoʻohuli i ka transcription a me qPCR ʻelua mau hopena i loko o ka pahu hoʻokahi, pono wale nō e hoʻohui i ka RNA template, nā kumu PCR kikoʻī a me ka RNase-Free ddH.2O.

◮Hiki i ka kit ke hoʻopaʻa i ka RNA viral a i ʻole ka huli ʻana i ka RNA.

◮Ke hoʻohana nei ka pahu i kahi mea hoʻohālikelike Foregene reverse transcription reagent a me Foregene HotStar Taq DNA Polymerase i hui pū ʻia me kahi ʻōnaehana kū hoʻokahi e hoʻomaikaʻi maikaʻi i ka pono amplification a me ka kikoʻī o ka pane.

◮ʻO ka ʻōnaehana hopena maikaʻi ʻoi aku ka nui o ka ʻike ʻana, ʻoi aku ka ikaika o ka wela, a ʻoi aku ka maikaʻi o ka hoʻomanawanui.

◮RT-qPCR maʻalahiTM(Hoʻokahi KaʻAnuʻu) - SYBR Green I kit hele mai me ka ROX internal reference dye, hiki ke hoʻohana ʻia no ka hoʻopau ʻana i ka hōʻailona hōʻailona a me nā hewa hōʻailona ma waena o nā pūnāwai, kahi kūpono i nā mea kūʻai aku e hoʻohana i nā ʻano like ʻole o nā mea kani PCR quantitative.

ikaika mua


Huahana Huahana

Huahana Huahana

Ke manaʻoʻiʻo nei mākou: ʻO ka hana hou ko mākou ʻuhane a me ko mākou ʻuhane.ʻO ke kūlana kiʻekiʻe ko mākou ola.Pono ka mea kūʻai aku i ko mākou Akua no ka laʻana manuahi no Kina High Sensitivity DNA/Rna One-Step Probe Rt-QpcrKit V2, I loko o kā mākou mau hana, ua loaʻa iā mākou nā hale kūʻai he nui ma Kina a ua lanakila kā mākou hoʻonā i ka mahalo mai nā mea kūʻai aku ma ka honua holoʻokoʻa.Welina mai i nā mea kūʻai hou a kahiko e hoʻokaʻaʻike mai iā mākou i nā pilina ʻoihana lōʻihi lōʻihi.
Ke manaʻoʻiʻo nei mākou: ʻO ka hana hou ko mākou ʻuhane a me ko mākou ʻuhane.ʻO ke kūlana kiʻekiʻe ko mākou ola.Pono ka mea kūʻai aku i ko mākou Akua noKina Taq DNA Polymerase, Qpcr, Hana ikaika e hoʻomau i ka holomua, hana hou i ka ʻoihana, hana i nā hana āpau i ka ʻoihana papa mua.Ke ho'āʻo nei mākou i kā mākou mea maikaʻi loa e kūkulu i ke kumu hoʻokele ʻepekema, e aʻo i ka ʻike ʻoihana nui, e hoʻomohala i nā lako hana kiʻekiʻe a me ke kaʻina hana, e hana i nā huahana maikaʻi kāhea mua, ke kumu kūʻai kūpono, ke kūlana kiʻekiʻe o ka lawelawe, ka hāʻawi wikiwiki ʻana, e hāʻawi iā ʻoe i ka waiwai hou.

Nā kikoʻī

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Nā ʻāpana pahu

Mahele I

Palena P1

Palena P2

6×DNA Loading Buffer

Mahele II

2× Lau PCR maʻalahiTMHui (UNG)

Nā hiʻohiʻona & pono

■ ʻAʻohe hoʻomaʻemaʻe DNA hoʻopau manawa a pipiʻi
■ He mea uuku
■ Maʻalahi-hiki ke hāʻawi ʻia ka laʻana i ka PCR a i ʻole ka hopena lysis me ka ʻoki ʻole a wili ʻole
■ 2 × Hoʻohui, hōʻemi i ka hāpana hoʻouka hewa a me ka pane ʻōnaehana manawa hoʻomākaukau
■ Hiki ke hoʻomākaukau i ka hoʻomākaukau wikiwiki i 10 mau minuke, hiki ke hoʻopau i ka hopena PCR i loko o 50 mau minuke.
■ Hiki ke hoʻopau ʻia ka hopena o ka throughput-lysis kiʻekiʻe ma ka pā 96-well PCR

Nā palena kime

Hoʻohana: genetically modified identification, genotyping, etc.
Laʻana: lau mea kanu
Ka hoʻopaʻa ʻana: ʻO ke anawaena 2-3mm (ke ala pololei), ke anawaena 5-7mm (ke ʻano lysis)
ʻIke ʻike: ʻāpana ʻāpana ≤1kb

Ka holo hana

Mea kanu Leaf Direct PCR Kit-UNG4

Electtropherogram

Mea kanu Leaf Direct PCR Kit-UNG5

ʻElua pule o ka ʻike ʻana o ka PCR Mix i nā hopena ma lalo o ke kūlana o ka PCR amplification product contamination.Ke manaʻoʻiʻo nei mākou: ʻO ka Innovation ko mākou ʻuhane a me ka ʻuhane.ʻO ke kūlana kiʻekiʻe ko mākou ola.Pono ka mea kūʻai aku i ko mākou Akua no Free sample for China High Sensitivity DNA/Rna One-Step Probe Rt-Qpcr Kit V2, I loko o kā mākou mau hana, ua nui nā hale kūʻai ma Kina a ua lanakila kā mākou mau hoʻonā i ka mahalo mai nā mea kūʻai aku ma ka honua holoʻokoʻa.Welina mai i nā mea kūʻai hou a kahiko e hoʻokaʻaʻike mai iā mākou i nā pilina ʻoihana lōʻihi lōʻihi.
Hāʻawi manuahi noKina Taq DNA Polymerase, Qpcr, Hana ikaika e hoʻomau i ka holomua, hana hou i ka ʻoihana, hana i nā hana āpau i ka ʻoihana papa mua.Ke ho'āʻo nei mākou i kā mākou mea maikaʻi loa e kūkulu i ke kumu hoʻokele ʻepekema, e aʻo i ka ʻike ʻoihana nui, e hoʻomohala i nā lako hana kiʻekiʻe a me ke kaʻina hana, e hana i nā huahana maikaʻi kāhea mua, ke kumu kūʻai kūpono, ke kūlana kiʻekiʻe o ka lawelawe, ka hāʻawi wikiwiki ʻana, e hāʻawi iā ʻoe i ka waiwai hou.

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