Me kēia huaʻōlelo i ka noʻonoʻo, ua lilo mākou i hoʻokahi o nā mea hana ʻenehana ʻoi loa, ʻoi aku ka maikaʻi, a me ka hoʻokūkū kumu kūʻai no ka High QualityKina Nucleic Acid Extraction Reagent, Rna Extraction Test Kits Nucleic Acid Extraction Reagent, Welina mai iā ʻoe e lilo i ʻāpana o mākou me kekahi i mea e maʻalahi ai kāu hui.ʻO mākou ka mea maʻamau i kāu hoa nui loa inā makemake ʻoe e loaʻa kāu ʻoihana liʻiliʻi ponoʻī.
Me kēia huaʻōlelo i ka noʻonoʻo, ua lilo mākou i hoʻokahi o nā mea hana ʻenehana hou loa, ʻoi aku ka maikaʻi, a me nā mea hoʻokūkū kumu kūʻai.Kina Nucleic Acid Extraction Reagent, ʻO ke ʻano ʻo Magnetic Bead, E hana mākou i kā mākou mea maikaʻi loa e launa pū me ʻoe e hilinaʻi ana i ka maikaʻi o ka papa kiʻekiʻe a me ke kumukūʻai hoʻokūkū a maikaʻi loa ma hope o ka lawelawe ʻana, e nānā pono i ka hui pū ʻana me ʻoe a hana i nā hoʻokō i ka wā e hiki mai ana!

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50 Mākaukau, 200 Mākaukau

Ke hoʻohana nei kēia pahu i ke kolamu spin a me ke ʻano i hoʻomohala ʻia e Foregene, hiki iā ia ke hoʻokaʻawale maikaʻi i ka RNA piha maʻemaʻe kiʻekiʻe a kiʻekiʻe mai nā cell i hoʻoulu ʻia ma nā papa 96, 24, 12, a me 6-well.

Hāʻawi ka pahu i kahi kolamu hoʻomaʻemaʻe DNA maikaʻi, hiki ke hoʻokaʻawale i ka supernatant a me ka cell lysate, hoʻopaʻa a wehe i ka DNA genomic.He maʻalahi ka hana a mālama manawa.

Hiki i ke kolamu RNA-wale ke hoʻopaʻa maikaʻi i ka RNA me kahi ʻano kū hoʻokahi.Hiki ke hana ʻia ka nui o nā laʻana i ka manawa like.

Nā ʻāpana pahu

*E ʻoluʻolu e ʻaʻahu i nā mīkina lima a e hana i nā hana pale i ka wā o ka hana ʻana no ka mea aia ʻo Buffer cRL1 a me Buffer RW1 i nā paʻakai chaotropic hoʻonāukiuki.

Nā hiʻohiʻona & pono

■ Hoʻohana ʻia ke kaʻina holoʻokoʻa ma ka lumi wela (15-25 ℃), me ka ʻauʻau hau a me ka centrifugation haʻahaʻa.
■ ʻO ka pahu holoʻokoʻa he RNase-Free, ʻaʻohe pono e hopohopo e pili ana i ka hōʻino ʻana o RNA.
■ Hoʻopaʻa pono ʻia ke kolamu hoʻomaʻemaʻe DNA i ka DNA, i hiki i ka pahu ke hoʻopau i ka ʻino DNA genomic me ka hoʻohui ʻole ʻana i ka DNase hou.
■ Loaʻa RNA kiʻekiʻe: Hiki i ke kolamu RNA-wale a me ka hoʻohālikelike kū hoʻokahi ke hoʻomaʻemaʻe pono i ka RNA.
■ ʻO ka wikiwiki wikiwiki: maʻalahi ka hana a hiki ke hoʻopau i nā minuke 11.
■ Palekana: ʻAʻole koi ʻia ka mea hoʻoponopono organik.
■ Ke kūlana kiʻekiʻe: ʻO ka RNA i hoʻomaʻemaʻe ʻia he maʻemaʻe kiʻekiʻe, ʻaʻohe protein a me nā mea haumia ʻē aʻe, a hiki ke hālāwai me nā hoʻokolohua like ʻole.

Ke noi pahu

He kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka nui o ka RNA mai nā cell moʻomeheu i nā papa 96, 24, 12, a me 6-well.

Ka holo hana

Diagram

Ua mālama ʻia ke kiʻi pākaukau agarose gel o ka Cell Total RNA Isolation Kit i nā helu like ʻole o luna, 20μl volume elution, e lawe i ka 2μl i hoʻomaʻemaʻe ʻia i ka RNA 1%.

Ka mālama ʻana a me ka mālama ʻana

Hiki ke mālama ʻia ka pahu no 12 mahina ma ka lumi wela (15–25 ℃) a i ʻole 2–8 ℃ no ka manawa lōʻihi (24 mau mahina).

Hiki ke mālama ʻia ʻo Buffer cRL1 ma 4 ℃ no 1 mahina ma hope o ka hoʻohui ʻana i ka 2-hydroxy-1-ethanethiol (koho).Kina Nucleic Acid Extraction Reagent, Rna Extraction Test Kits Nucleic Acid Extraction Reagent, Welina mai iā ʻoe e lilo i ʻāpana o mākou me kekahi i mea e maʻalahi ai kāu hui.ʻO mākou ka mea maʻamau i kāu hoa nui loa inā makemake ʻoe e loaʻa kāu ʻoihana liʻiliʻi ponoʻī.
High Quality Kina Nucleic Acid Extraction Reagent,ʻO ke ʻano ʻo Magnetic Bead, E hana mākou i kā mākou mea maikaʻi loa e launa pū me ʻoe e hilinaʻi ana i ka maikaʻi o ka papa kiʻekiʻe a me ke kumukūʻai hoʻokūkū a maikaʻi loa ma hope o ka lawelawe ʻana, e nānā pono i ka hui pū ʻana me ʻoe a hana i nā hoʻokō i ka wā e hiki mai ana!