Kiʻi Hoʻomaʻemaʻe Rna Kina kiʻekiʻe (Kolana Milo)
Hoʻohiki kā mākou paʻa i nā mea hoʻohana a pau ma nā huahana papa mua a me nā hoʻonā me ke kōkua ma hope o ke kūʻai ʻana.Hoʻokipa maikaʻi mākou i kā mākou mau mea kūʻai maʻamau a me nā mea hou e hui pū me mākou no High Quality China Rna Purification Kit (Spin Column), me ka hoʻohana ʻana i ka manaʻo mau loa o ka "hoʻomaikaʻi mau ʻana i ka maikaʻi, ʻoluʻolu ka mea kūʻai aku", ʻike mākou i kā mākou mea maikaʻi loa he palekana a kuleana a ʻo kā mākou huahana a me nā hoʻonā e kūʻai maikaʻi loa ʻia ma kou home a ma waho.
Hoʻohiki kā mākou paʻa i nā mea hoʻohana a pau ma nā huahana papa mua a me nā hoʻonā me ke kōkua ma hope o ke kūʻai ʻana.Hoʻokipa maikaʻi mākou i kā mākou mau mea kūʻai maʻamau a hou e hui pū me mākou noKina Rna Wehe, Hoao DNA, Welina mai i kekahi o kāu mau nīnau a me kou hopohopo no kā mākou waiwai.Manaʻo mākou i ka hoʻokumu ʻana i kahi pilina ʻoihana lōʻihi me ʻoe i ka wā e hiki mai ana.E kelepona mai iā mākou i kēia lā.ʻO mākou ka hoa ʻoihana mua iā ʻoe iho!
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50 Mākaukau, 200 Mākaukau
Ke hoʻohana nei kēia pahu i ke kolamu spin a me ke ʻano i hoʻomohala ʻia e Foregene, hiki iā ia ke hoʻokaʻawale maikaʻi i ka RNA piha maʻemaʻe kiʻekiʻe a kiʻekiʻe mai nā cell i hoʻoulu ʻia ma nā papa 96, 24, 12, a me 6-well.
Hāʻawi ka pahu i kahi kolamu hoʻomaʻemaʻe DNA maikaʻi, hiki ke hoʻokaʻawale i ka supernatant a me ka cell lysate, hoʻopaʻa a wehe i ka DNA genomic.He maʻalahi ka hana a mālama manawa.
Hiki i ke kolamu RNA-wale ke hoʻopaʻa maikaʻi i ka RNA me kahi ʻano kū hoʻokahi.Hiki ke hana ʻia ka nui o nā laʻana i ka manawa like.
Nā ʻāpana pahu
haku mele | RE-03111 | RE-03114 |
50 T | 200 T | |
Mālama cRL1* | 25 mL | 100 mL |
Mālama cRL2 | 15 mL | 60 mL |
Hoʻopaʻa RW1* | 25 mL | 100 mL |
Hoʻopaʻa RW2 | 24 mL | 96 mL |
RNase-Free ddH2O | 10 mL | 40 mL |
RNA-Kola wale nō | 50 | 200 |
Kona Hoomaemae DNA | 50 | 200 |
Aʻo | 1 | 1 |
*E ʻoluʻolu e ʻaʻahu i nā mīkina lima a e hana i nā hana pale i ka wā o ka hana ʻana no ka mea aia ʻo Buffer cRL1 a me Buffer RW1 i nā paʻakai chaotropic hoʻonāukiuki.
Nā hiʻohiʻona & pono
■ Hoʻohana ʻia ke kaʻina holoʻokoʻa ma ka lumi wela (15-25 ℃), me ka ʻauʻau hau a me ka centrifugation haʻahaʻa.
■ ʻO ka pahu holoʻokoʻa he RNase-Free, ʻaʻohe pono e hopohopo e pili ana i ka hōʻino ʻana o RNA.
■ Hoʻopaʻa pono ʻia ke kolamu hoʻomaʻemaʻe DNA i ka DNA, i hiki i ka pahu ke hoʻopau i ka ʻino DNA genomic me ka hoʻohui ʻole ʻana i ka DNase hou.
■ Loaʻa RNA kiʻekiʻe: Hiki i ke kolamu RNA-wale a me ka hoʻohālikelike kū hoʻokahi ke hoʻomaʻemaʻe pono i ka RNA.
■ ʻO ka wikiwiki wikiwiki: maʻalahi ka hana a hiki ke hoʻopau i nā minuke 11.
■ Palekana: ʻAʻole koi ʻia ka mea hoʻoponopono organik.
■ Ke kūlana kiʻekiʻe: ʻO ka RNA i hoʻomaʻemaʻe ʻia he maʻemaʻe kiʻekiʻe, ʻaʻohe protein a me nā mea haumia ʻē aʻe, a hiki ke hālāwai me nā hoʻokolohua like ʻole.
Ke noi pahu
He kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka nui o ka RNA mai nā cell moʻomeheu i nā papa 96, 24, 12, a me 6-well.
Ka holo hana
Diagram
Ua mālama ʻia ke kiʻi pākaukau agarose gel o ka Cell Total RNA Isolation Kit i nā helu like ʻole o luna, 20μl volume elution, e lawe i ka 2μl i hoʻomaʻemaʻe ʻia i ka RNA 1%.
Ka mālama ʻana a me ka mālama ʻana
Hiki ke mālama ʻia ka pahu no 12 mahina ma ka lumi wela (15–25 ℃) a i ʻole 2–8 ℃ no ka manawa lōʻihi (24 mau mahina).
Hiki ke mālama ʻia ka Buffer cRL1 ma 4 ℃ no 1 mahina ma hope o ka hoʻohui ʻana i ka 2-hydroxy-1-ethanethiol (koho).
Hoʻohiki kā mākou paʻa i nā mea hoʻohana a pau ma nā huahana papa mua a me nā hoʻonā me ke kōkua ma hope o ke kūʻai ʻana.Hoʻokipa maikaʻi mākou i kā mākou mau mea kūʻai maʻamau a me nā mea hou e hui pū me mākou no High Quality China Rna Purification Kit (Spin Column), me ka hoʻohana ʻana i ka manaʻo mau loa o ka "hoʻomaikaʻi mau ʻana i ka maikaʻi, ʻoluʻolu ka mea kūʻai aku", ʻike mākou i kā mākou mea maikaʻi loa he palekana a kuleana a ʻo kā mākou huahana a me nā hoʻonā e kūʻai maikaʻi loa ʻia ma kou home a ma waho.
Kūlana KiʻekiʻeKina Rna Wehe, Ho'āʻo DNA, Welina mai i kāu mau nīnau a me kāu hopohopo no kā mākou waiwai.Manaʻo mākou i ka hoʻokumu ʻana i kahi pilina ʻoihana lōʻihi me ʻoe i ka wā e hiki mai ana.E kelepona mai iā mākou i kēia lā.ʻO mākou ka hoa ʻoihana mua iā ʻoe iho!