Lnc-RT Heroᵀᴹ I(Me gDNase)(Super Premix no ke kaula cDNA synthesis mua mai lncRNA)
Nā kikoʻī
25×20μl Rxns, 50×20μl Rxns
ʻO Lnc-RT HeroTM I (Me gDNase) he ʻōnaehana hoʻololi i hoʻomohala kūikawā no ka lncRNA e hoʻoneʻe koke i ka ʻino DNA genomic.Hiki iā 5×gDNase Mix ke wehe koke i ke koena genome ma RNA ma 42°C no 2 mau minuke, me ka pale pono ʻana i ka hoʻopili ʻana o ka genome ma nā hopena qPCR.
Aia ka 5×L-RT HeroTM Mix i ka Foregene LncRNA Reverse Transcriptase i hoʻomohala kūikawā ʻia e Foregene, he ʻano hou o ka reverse transcriptase kūikawā no ka lncRNA a me nā laʻana paʻakikī RNA lōʻihi, me ka pilina RNA ikaika a me ka maikaʻi o ka hoʻopaʻa hoʻopaʻa ʻana kiʻekiʻe.ʻO ka ʻōnaehana i hoʻopaʻa ʻia e ʻoi aku ka wikiwiki o ka transcription rate a hiki ke maʻalahi i ka unuhi ʻana i nā mamana RNA me ka maʻiʻo GC kiʻekiʻe a me ka hana lua paʻakikī.Hiki ke hoʻopau ʻia ka strand cDNA synthesis mua ma 42°C i 15 mau minuke.
Nā ʻāpana pahu
| 5× Huihui gDNase |
| 5× L-RT HeroTM Mix |
| RNase-Free ddH2O |
| Nā kuhikuhi |
Nā hiʻohiʻona & pono
■ʻO ka hiki ke wehe i ka gDNA, hiki ke wehe i ka gDNA i ka template i loko o 2 mau minuke.
■ Hiki iā ia ke hoʻohuli maikaʻi i ka unuhi ʻana o ka lncRNA.Ke hoʻokau ʻia ka huahana hoʻohuli i ka qPCR, ʻoi aku ka haʻahaʻa o ka waiwai Ct ma mua o ka ʻōnaehana hoʻololi maʻamau.
■ He 15 mau minuke wale no ka hoopau ana i ka synthesis o ke kaula cDNA mua.
■ Nā hiʻohiʻona paʻakikī: hiki ke hoʻohuli ʻia nā hiʻohiʻona me ka maʻiʻo GC kiʻekiʻe a me ka hana lua paʻakikī me ka hana kiʻekiʻe.
■ Kiʻekiʻe-sensitivity reverse transcription nenoaiu, pg-level templates hiki no hoi ke kiʻekiʻe kiʻekiʻe cDNA.
■ He kiʻekiʻe ka wela o ka ʻōnaehana hoʻololi ʻana, ʻo 42 ka wela o ka hopena maikaʻi loa℃, a loaʻa mau ka hana transcription hope maikaʻi ma 50℃.
Ke noi pahu
lncRNA pili ana i ka helu helu.
lncRNA hoʻomākaʻikaʻi nui.
Hiki iā ia ke nānā wikiwiki a pololei i ka trace RNA e like me RNA virus.
Hoʻololi i ka unuhi ʻana o nā mamana RNA me ka maʻiʻo GC kiʻekiʻe a i ʻole ka hana lua paʻakikī.
Ka holo hana
Ka mālama ʻana a me ka mālama ʻana
Pono e mālama ʻia ka pahu ma -20°C. E mālama i ka huahana i loko o ka pahu hau wela mau ma -20°C ma hope koke iho o ka loaʻa ʻana.Inā kūpono nā kūlana mālama, ʻaʻole e hoʻohaʻahaʻa ka huahana i kekahi hana i ka manawa o ka makahiki 1.
Ke alakaʻi hoʻoponopono pilikia
The following is an analysis of the problems that may be encountered in the use of Lnc-RT HeroTM I (With gDNase) series kits, hoping to be helpful to your experiments. In addition, we have dedicated technical support to help you with other experimental or technical problems beyond the operating instructions and questions. If you have any needs, please contact us: 028-83361257 or E-mail: Tech@foregene.com.
Non-hoʻonui kikoʻī
1. Hoʻolālā kumu kumu kūpono ʻole
Manaʻo: E hoʻolālā i nā kumu mua e like me nā kumu kumu hoʻolālā.
2. Nā koena genome.
Manaʻo Manaʻo: E hōʻoia i ka pae mua o ka hoʻoneʻe ʻana o ka gDNA i ka wela o ka hopena he 42°C, a hiki ke hoʻonui ʻia ka manawa pane i 5min.
ʻAʻohe hōʻailona hoʻonui e RT-qPCR
1. Hoʻohaʻahaʻa ʻia ka RNA.
Manaʻo Manaʻo: Pono ka mea no ka unuhi ʻana i ka RNA e like me ka mea hiki, a pono e hoʻohana ʻia ka RNA kiʻekiʻe a me ka maʻemaʻe kiʻekiʻe.
2. Loaʻa i ka RNA nā mea pale.
Manaʻo Manaʻo: Hoʻokomo ʻia nā mea hoʻopiʻi transcription hoʻohuli i ka SDS, nā paʻakai guanidine, EDTA, a me nā mea ʻē aʻe. Manaʻo ʻia e holoi i ka RNA precipitate me 70% ethanol e wehe i nā mea paʻa.
3. Nā pilikia hoʻolālā kumu.
Manaʻo Manaʻo: E like me ke kumu hoʻolālā kumu, hoʻolālā hou i nā primers no ka nānā ʻana.
Hōʻike ʻia ka pūʻali i hoʻopaʻa ʻia i ka mana blank
1. Ka hoʻohaumia ʻana i nā mea hana a i ʻole reagents.
Manaʻo: Pono e hoʻokuʻu ʻia nā reagents a i ʻole nā mea hana no ka hoʻokolohua.E akahele a akahai i ka lawelawe ʻana e pale aku i nā laʻana DNA mai ka omo ʻana i loko o ka pipette a i ʻole ke kahe ʻana mai loko mai o ka paipu centrifuge.
2. Ua ho'opō'ino 'ia ma ka ho'omākaukau 'ana i ka PCR reaction system.
Manaʻo: E mālama pono i ka wā e lawelawe ai, e like me: ke komo ʻana i nā mīkina lima latex, me ka hoʻohana ʻana i nā ʻōlelo aʻoaʻo kānana.E hoʻohana i ka Mix PCR Real Time i loko o kahi ʻōnaehana pale-poino.
3. Hoʻohaʻahaʻa ʻia nā primers.
Manaʻo Manaʻo: E hoʻohana i ka electrophoresis SDS-PAGE e ʻike inā ua hoʻohaʻahaʻa ʻia nā primers, a hoʻololi me nā primers hou no nā hoʻokolohua ʻike fluorescence.
Papa kuhikuhi:
Lnc-RT HeroTM I(Me gDNase)(Super Premix no ke kaula mua cDNA synthesis mai lncRNA) Palapala A'o.
