ʻO nā pahu hoʻokaʻawale plasmid DNA ka hana ʻana i ke kolamu wikiwiki a maʻalahi hoʻi
ʻO ka wehewehe ʻana o ka pahu:
E hoʻomaʻemaʻe wikiwiki i ka DNA plasmid kiʻekiʻe mai nā bacteria i hoʻololi ʻia no nā hoʻokolohua molecular biology maʻamau e like me ka hoʻololi ʻana a me ka digestion enzyme.
◮Wehe i ka RNA me ka hoʻohui ʻole i ka RNase
◮ʻoluʻolu—hana ʻia ka centrifugation ma ka lumi wela
◮wikiwiki- hiki ke hoʻopau i ka hana i loko o 20 mau minuke
◮Palekana—ʻaʻole i hoʻohana ʻia ka mea hoʻoponopono organik
◮Maʻemaʻe kiʻekiʻe—OD260/280≈1.7-1.9
No ka hoʻokumu ʻana i ka waiwai nui no nā mea kūʻai aku ʻo kā mākou ʻoihana ʻoihana;ʻO ka ulu ʻana o ka mea kūʻai aku ʻo kā mākou hana hana no ka plasmid dna isolation kits lumi lumi hana spin kolamu wikiwiki a maʻalahi, manaʻo mākou e hiki i kahi hui hoihoi, kipi a hoʻomaʻamaʻa maikaʻi ke hoʻonohonoho i nā pilina ʻoihana maikaʻi loa me ʻoe.E hoʻomanaʻo i ka manaʻo ʻaʻohe kumu kūʻai e launa pū me mākou no nā ʻike hou aku.
No ka hoʻokumu ʻana i ka waiwai nui no nā mea kūʻai aku ʻo kā mākou ʻoihana ʻoihana;ʻO ka ulu ʻana o ka mea kūʻai aku ʻo kā mākou hana hanaKina DNA Extract, Hoʻokaʻawale Dna, Mai ka manawa mau, ke hoʻomau nei mākou i ka "wehe a me ka pololei, kaʻana like, ka ʻimi ʻana i ka maikaʻi, a me ka hoʻokumu ʻana i ka waiwai" waiwai, pili i ka "kūpono a me ka maikaʻi, kālepa-oriented, ala maikaʻi loa, valve maikaʻi loa" ʻoihana ʻikepili.Me ko mākou honua holoʻokoʻa he mau lālā a me nā hoa hana e hoʻomohala i nā wahi ʻoihana hou, nā kumu waiwai maʻamau.Aloha nui mākou a hui pū mākou i nā kumuwaiwai honua, e wehe ana i kahi ʻoihana hou me ka mokuna.
Nā wehewehe kime
Hiki ke unuhi ʻia a hoʻomaʻemaʻe ʻia nā huahana nucleic acid o FOREGENE mai nā kumu like ʻole a me nā mea hoʻohālike paʻakikī.Hoʻokumu ʻia nā huahana DNA extraction series ma nā kolamu hoʻomaʻemaʻe ʻana o ka membrane silica DNA wale nō a me nā reagents formula kūikawā hiki ke hoʻomaʻemaʻe i ka DNA kiʻekiʻe mai nā ʻano laʻana.
Hoʻohana ka General Plasmid Mini Kit i ka ʻenehana kolamu hoʻomaʻemaʻe DNA-Only a me kahi formula lysis SDS kūpono e loaʻa ai ka DNA plasmid kiʻekiʻe mai ka bacteria i loko o 20 mau minuke.ʻO ka DNA-Only silica gel membrane i hoʻohana ʻia i loko o ke kolamu spin he mea kūʻokoʻa hou ia o FOREGENE, hiki iā ia ke hoʻopili i ka DNA me ka maikaʻi a kikoʻī, a hoʻoneʻe i ka RNA, nā protein haumia, nā ion a me nā mea hoʻohui ʻē aʻe i loko o nā cell i ka nui loa.ʻO ka mana hoʻopaʻa paʻa kiʻekiʻe o ka DNA plasmid o ka kolamu hoʻomaʻemaʻe DNA-Only he 60μg DNA.ʻO ka nui o ka DNA plasmid i unuhi maoliʻia e pili ana i ka nui o ka hopena bacteria, ka helu o nā kope plasmid (kope kiʻekiʻe, kope haʻahaʻa), nā kānana, nā kūlana moʻomeheu a me nā mea'ē aʻe.
Nā kikoʻī
| Puke Mini Plasmid | |||
| Kiko maʻiʻo | DE-01001 | DE-01002 | DE-01003 |
| 50T | 100T | 250T | |
| Palena S1 | 10ml | 20ml | 50ml |
| Palena S2 | 10ml | 20ml | 50ml |
| Hoʻopaʻa S3 | 25ml | 50ml | 125ml |
| Hoopaa PW | 25ml | 50ml | 125ml |
| Mālama WB2 | 15ml | 30ml | 75ml |
| Hoʻopaʻa EB | 10ml | 20ml | 50ml |
| DNA-wale wale ke kolamu | 50 | 100 | 250 |
| Manual | 1 | 1 | 1 |
Nā hiʻohiʻona & pono
- ʻAʻohe RNase contamination: ʻO ka DNA-Only Column i hāʻawi ʻia e ka kit e hiki ai ke wehe i ka RNA i loko o ka DNA plasmid me ka hoʻohui ʻole ʻana i ka RNase i ka wā o ka hoʻokolohua, e pale ana i ka hoʻopōʻino ʻia o ka hale hana e ka exogenous RNase.
- Ka wikiwiki wikiwiki: Hiki ke hoʻopau i ka unuhi ʻana o Plasmid DNA i loko o 20 mau minuke.
- Palekana: ʻAʻole koi ʻia ka unuhi ʻana o ka reagent.
- Kiʻekiʻe kiʻekiʻe: ʻO ka plasmid i loaʻa ma ka unuhi ʻana ʻaʻohe RNA, ʻaʻohe RNase a me ka haʻahaʻa haʻahaʻa haʻahaʻa, hiki ke hoʻokō i nā koi o nā hoʻokolohua like ʻole.
Ke noi pahu
ʻO ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka DNA Plasmid no ka maʻi bacteria Gram-negative.
Ka holo hana
Diagram
Ka mālama ʻana a me ka mālama ʻana
Hiki ke mālama ʻia kēia kit no 12 mau mahina ma lalo o nā kūlana maloʻo ma ke ana wela (15-25 ℃);inā pono e mālama ʻia no ka manawa lōʻihi, hiki ke mālama ʻia ma 2-8 ℃.
'Ōlelo Aʻo: Inā mālama ʻia ma kahi wela haʻahaʻa, hiki i ka hāʻina ke hiki i ka ua.Ma mua o ka hoʻohana ʻana, e hōʻoia e waiho i ka hopena i loko o ka pahu i ka lumi wela no kekahi manawa.Inā pono, e hoʻomaʻamaʻa mua iā ia i loko o kahi ʻauʻau wai 37 ℃ no 10 mau minuke e hoʻoheheʻe i ka wai, a hui pū ma mua o ka hoʻohana ʻana.
No ka hoʻokumu ʻana i ka waiwai nui no nā mea kūʻai aku ʻo kā mākou ʻoihana ʻoihana;ʻO ka ulu ʻana o ka mea kūʻai aku ʻo kā mākou hana hana no Wholesale Dealers o Kina 96Automed Nucleic Acid Extraction System for PCR Sampling Preparation, Ke manaʻoʻiʻo nei mākou e hiki i kahi hui hoihoi, kipi a hoʻomaʻamaʻa maikaʻi ke hoʻonohonoho i nā pilina ʻoihana maikaʻi loa me ʻoe.E hoʻomanaʻo i ka manaʻo ʻaʻohe kumu kūʻai e launa pū me mākou no nā ʻike hou aku.
Nā mea kūʻai kūʻai nui oKina DNA Extract, DNA Isolation, Mai ka manawa mau, ke hoʻomau nei mākou i ka "wehe a me ka pololei, kaʻana like e loaʻa, ka ʻimi ʻana i ka maikaʻi, a me ka hana ʻana i nā waiwai" waiwai, pili i ka "kūpono a me ka maikaʻi, kālepa-oriented, ala maikaʻi loa , valve maikaʻi loa" ʻoihana philosophy.Me ko mākou honua holoʻokoʻa he mau lālā a me nā hoa hana e hoʻomohala i nā wahi ʻoihana hou, nā kumu waiwai maʻamau.Aloha nui mākou a hui pū mākou i nā kumuwaiwai honua, e wehe ana i kahi ʻoihana hou me ka mokuna.
