ATM/CSP11 Hoʻopaʻa Wai ʻElua
ʻO ka wehewehe ʻana o ka pahu:
Ma muli o ke kumu o ka hoʻohui pū ʻana o nā waihona DNA, ua hoʻohana ʻia ka ʻimi ʻalani ATM a me ka ʻimi ʻōmaʻomaʻo CSP11 e hoʻohui i ke kaʻina pahuhopu DNA i loko o ka nucleus, a ua nānā ʻia ka ʻike kūlana gene i loko o ka nucleus a nānā ʻia ma lalo o ka microscope fluorescence.
wehewehe
Ma muli o ke kumu o ka hoʻohui pū ʻana o nā waihona DNA, ua hoʻohana ʻia ka ʻimi ʻalani ATM a me ka ʻimi ʻōmaʻomaʻo CSP11 e hoʻohui i ke kaʻina pahuhopu DNA i loko o ka nucleus, a ua nānā ʻia ka ʻike kūlana gene i loko o ka nucleus a nānā ʻia ma lalo o ka microscope fluorescence.
Nā ʻāpana pahu
| ʻāpana | Nā kikoʻī | ||
| 5Nā hoʻāʻo | 10Nā hoʻāʻo | 20Nā hoʻāʻo | |
| ATM/CSP11ʻO ka ʻimi kala ʻelua | 50μl | 100μl | 200μl |
Ke noi pahu
Hoʻohana ʻia kēia pahu no ka ʻike pono ʻana i ke ʻano like ʻole o ke kanaka ATM (11q22) gene i loko o nā laʻana cytological a i ʻole nā palapala kikoo paraffin-embedded in vitro.
Nā koi hoʻohālike
No ka iwi iwi a i ʻole nā ʻāpana koko ʻokoʻa, mālama ʻia nā laʻana hou i hoʻopaʻa ʻole ʻia ma 4 ℃ no nā hola he 24, a mālama ʻia ka hoʻokuʻu ʻana o ka cell paʻa ma-20 ℃.
Ka mālama ʻana a me ka mālama ʻana
Mālama ʻia ma -20 ℃ ± 5 ℃ i ka pōʻeleʻele, kūpono no 12 mau mahina.ʻO ka halihali ma lalo o 8 ℃.
