ʻO RARA Pālua Weheʻe Wehe Kaʻawale
ʻO ka wehewehe ʻana o ka pahu:
Ma muli o ke kumu o ka hoʻohui pū ʻana o nā kumu DNA, ua hoʻohana ʻia ka ʻimi ʻālani RARA a me ka ʻimi ʻōmaʻomaʻo RARA e hoʻohui pū me ke kaʻina pahuhopu DNA i loko o ka nucleus, a ua nānā ʻia ka ʻike kūlana gene i loko o ka nucleus ma lalo o ka microscope fluorescence.
wehewehe
Ma muli o ke kumu o ka hoʻohui pū ʻana o nā kumu DNA, ua hoʻohana ʻia ka ʻimi ʻālani RARA a me ka ʻimi ʻōmaʻomaʻo RARA e hoʻohui pū me ke kaʻina pahuhopu DNA i loko o ka nucleus, a ua nānā ʻia ka ʻike kūlana gene i loko o ka nucleus ma lalo o ka microscope fluorescence.
Nā ʻāpana pahu
| ʻāpana | Nā kikoʻī | ||
| 5 Hoao | 10 Hoao | 20 Hoao | |
| ʻO RARA Pālua Weheʻe Wehe Kaʻawale | 50μl | 100μl | 200μl |
Ke noi pahu
Hoʻohana ʻia kēia kit no ka ʻike pono ʻana i ke ʻano like ʻole o ka gene RARA kanaka i loko o nā laʻana cytological a i ʻole nā palapala kikoo paraffin-embedded in vitro.
Nā koi hoʻohālike
-ʻano kikoʻī
Ua hoʻopaʻa ʻia nā ʻano kikoo paraffin i hoʻopaʻa ʻia i loko o 10% neutral buffered formalin, a ʻo ka mānoanoa ʻāpana ma waena o 3 a me 5 µm.
-ʻĀpana kelepona
No ka iwi iwi a i ʻole nā laʻana koko ʻaoʻao, pono e mālama ʻia nā laʻana hou i hoʻopaʻa ʻole ʻia ma 4 ° C no nā hola he 24, a pono e mālama ʻia ka hoʻokuʻu ʻana o ke kelepona paʻa ma -20°C.
Ka mālama ʻana a me ka mālama ʻana
Mālama ʻia ma -20 ℃ ± 5 ℃ i ka pōʻeleʻele, kūpono no 12 mau mahina.ʻO ka halihali ma lalo o 8 ℃.
