DAPI Counterstain
ʻO ka wehewehe ʻana o ka pahu:
ʻO ka mea nui o ka DAPI re-staining solution he 4-phenylindole, hiki ke komo i ka membrane cell a hoʻopaʻa ikaika i ka DNA.
wehewehe
ʻO ka mea nui o ka DAPI re-staining solution he 4-phenylindole, hiki ke komo i ka membrane cell a hoʻopaʻa ikaika i ka DNA.Hiki ke ʻike ʻia ka nucleus fluorescent polū ma lalo o ka hauʻoli o ke kukui ultraviolet ma lalo o ka microscope fluorescence.
Nā ʻāpana pahu
| ʻāpana | Nā kikoʻī | ||
| 5Nā hoʻāʻo | 10Nā hoʻāʻo | 20Nā hoʻāʻo | |
| DAPIKūʻē | 50μl | 200μl | 500μl |
Ke noi pahu
Hoʻohana ʻia no ke kala ʻana i ka nuclei.
Nā koi hoʻohālike
ʻAno hoʻohālike pili: ʻiʻo a i ʻole nā mea hoʻohālike kelepona i ʻike ʻia e ka hybridization in situ.
Nā palena
Hoʻopili wale ʻia kēia ʻano hana i ka hoʻopaʻa ʻia ʻana o nā laʻana kikoʻī a i ʻole nā lamehana cell i ʻike ʻia e ka hybridization in situ, a ʻaʻole pili i ke kala ʻana o nā laʻana i ʻike ʻia e nā ʻano hana ʻē aʻe.
Ka mālama ʻana a me ka mālama ʻana
Mālama ʻia ma -20 ℃ ± 5 ℃ i ka pōʻeleʻele, kūpono no 12 mau mahina.ʻO ka halihali ma lalo o 8 ℃.
