Koko RNA Isolation Kit
ʻO ka wehewehe ʻana o ka pahu:
Cat.No.RE-04011/04013
No ka hoʻomaʻemaʻe RNA a pau mai ke koko holoʻokoʻa 104 ≤ Nā Koko Keʻokeʻo ≤ 107
Hoʻokaʻawale wikiwiki a hoʻomaʻemaʻe i ke koko RNA mai nā keʻokeʻo keʻokeʻo.
-ʻAʻole pono e hopohopo e pili ana i ka hōʻino ʻana o RNA.ʻO ka pahu holoʻokoʻa he RNase-Free
-Maʻalahi—ua hoʻopau ʻia nā hana a pau ma ka lumi wela
-Fast—hiki ke hoʻopau i ka hana i loko o 20 mau minuke
-Ka hua RNA kiʻekiʻe: ʻO ke kolamu RNA-wale nō a me ke ʻano hoʻohālikelike hiki ke hoʻomaʻemaʻe pono i ka RNA
-Pale—ʻaʻole hoʻohana ʻia ka mea hoʻoheheʻe organik
-ʻO ka nui o ka hoʻoheheʻe ʻana o ka laʻana-a hiki i 200μl nā laʻana hiki ke hana i kēlā me kēia manawa.
-Ke kūlana kiʻekiʻe-ʻo ka RNA i hoʻomaʻemaʻe ʻia he maʻemaʻe loa, ʻaʻohe protein a me nā mea haumia ʻē aʻe, a hiki ke hālāwai me nā noi hoʻokolohua like ʻole.
Nā wehewehe
Hoʻohana ka kit i ke kolamu spin a me ka formula i hoʻomohala ʻia e kā mākou hui, hiki iā ia ke lawe maikaʻi i ka RNA holoʻokoʻa maʻemaʻe kiʻekiʻe a kiʻekiʻe mai ke koko holoʻokoʻa anticoagulated.Hāʻawi ka pahu i ka lysate ʻulaʻula ʻulaʻula (Buffer RCL), hiki iā ia ke hoʻoheheʻe wikiwiki a me ka maikaʻi i nā ʻāpana koko ʻulaʻula a mālama i nā keʻokeʻo keʻokeʻo.Hiki i ka DNA-Cleaning Colunm ke hoʻokaʻawale maʻalahi i ka supernatant a me nā cell lysates a adsorb a wehe i ka DNA genomic.He maʻalahi ka hana a mālama manawa;Hiki i ka RNA-only Column ke hoʻopaʻa maikaʻi i ka RNA, a me kahi ʻano ʻokoʻa, hiki iā ia ke hana i ka nui o nā laʻana i ka manawa like.
ʻO ka ʻōnaehana holoʻokoʻa RNase-Free e hana i ka RNA i unuhi ʻole ʻia;ʻO ka Buffer RW1 a me ka Buffer RW2 ʻōnaehana holoi holoi e hoʻolilo i ka RNA i loaʻa me ka ʻole o ka protein, DNA, nā ion a me ka haumia hui.
Kiko kiko
| Koko Huina RNA Isolation Kit | ||
| haku mele | RE-04011 | RE-04013 |
| 50 manawa | 200 manawa | |
| Hoʻopaʻa RCL (10×) | 52.5 mL | 210mL |
| Mālama BRL1* | 30mL | 120mL |
| Mālama BRL2 | 18mL | 66mL |
| Hoʻopaʻa RW1* | 25mL | 100mL |
| Hoʻopaʻa RW2 | 24mL | 96mL |
| RNase-Free ddH2 O | 10mL | 40mL |
| Kolu RNA wale nō | 50 sets | 200 sets |
| Kona Hoomaemae DNA | 50 sets | 200 sets |
| manual | 1 kope | 1 kope |
Nā hiʻohiʻona & pono
-ʻAʻole pono e hopohopo e pili ana i ka hōʻino ʻana o RNA.ʻO ka pahu holoʻokoʻa he RNase-Free.
-Maʻalahi—ua hoʻopau ʻia nā hana a pau ma ka lumi wela.
-Fast—hiki ke hoʻopau i ka hana i loko o 20 mau minuke.
-Ka hua RNA kiʻekiʻe: ʻO ke kolamu RNA-wale nō a me ke ʻano hoʻohālikelike hiki ke hoʻomaʻemaʻe maikaʻi i ka RNA.
-Pale—ʻaʻole hoʻohana ʻia ka mea hoʻoheheʻe organik.
-ʻO ka nui o ka hoʻoheheʻe ʻana o ka laʻana-a hiki i 200μl nā laʻana hiki ke hana i kēlā me kēia manawa.
-Ke kūlana kiʻekiʻe-ʻo ka RNA i hoʻomaʻemaʻe ʻia he maʻemaʻe loa, ʻaʻohe protein a me nā mea haumia ʻē aʻe, a hiki ke hālāwai me nā noi hoʻokolohua like ʻole.
Nā palena kime
Ke noi pahu:
He kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka RNA āpau mai ke koko holoʻokoʻa mammalian.
Ka holo hana
Nā kūlana mālama
Pono e mālama ʻia ka Buffer RCL (10×) ma 2-8 ℃;Hiki ke mālama ʻia nā ʻāpana ʻē aʻe o ka pahu ma kahi mahana wela (15-25 ℃) ma lalo o nā kūlana maloʻo, a hiki ke mālama ʻia no 12 mau mahina.Hiki ke mālama ʻia ʻo Buffer BRL1 ma 4 ℃ no 1 mahina ma hope o ka hoʻohui ʻana i ka β-mercaptoethanol (koho).
'Ōlelo Aʻo: Inā mālama ʻia ma kahi wela haʻahaʻa, hiki i ka hāʻina ke hiki i ka ua.E hōʻoia e waiho i ka hopena i loko o ka pahu ma kahi mahana wela no kekahi manawa ma mua o ka hoʻohana ʻana.Inā pono, e hoʻomaʻamaʻa mua i ka wai ʻauʻau 37 ° C no 10 mau minuke e hoʻoheheʻe i ka precipitate, a hui maikaʻi ma mua o ka hoʻohana ʻana.
Nā alakaʻi no ka nānā ʻana i nā pilikia
The following is an analysis of the problems that might be encountered in the extraction of viral RNA. We wish it would be helpful to your experiment. In addition, for other experimental or technical problems other than operating instructions and problem analysis, we have dedicated technical support to help you. Contact us if you need at : 028-83361257or E-mail：Tech@foregene.com。
ʻAʻole hiki ke unuhi ʻia ka RNA a i ʻole he haʻahaʻa ka hua o ka nucleic acid
Nui nā mea e pili ana i ka hoʻihoʻi ʻana, e like me: sample RNA content, ala o ka hana, elution volume, etc.
Ka nānā 'ana i nā kumu ma'amau:
1.Ice bath a haʻahaʻa-mehana (4 ° C) centrifugation i ka wā hana.
Manaʻo: Hana wela lumi (15-25 ° C), ʻaʻole ʻauʻau hau a me ka centrifuge wela haʻahaʻa.
2. Ka mālama pono ʻole ʻana i ka laʻana a i ʻole ka waiho ʻana o ka laʻana no ka lōʻihi loa.
Manaʻo Manaʻo: E kūʻai i nā laʻana ma -80 ° C a i ʻole i loko o ka hau hau i loko o ka naikokene wai, a pale i ka hoʻohana pinepine ʻana i ka hau hau;e ho'āʻo e hoʻohana i nā laʻana i hōʻiliʻili hou ʻia no ka unuhi ʻana o RNA.
3.Insufficient hāpana lysis
Manaʻo Manaʻo: E ʻoluʻolu e ʻoluʻolu e hui pū ʻia ka hāpana a me ka hopena hana (Linear Acrylamide) a hoʻomoʻi ʻia no 10 min ma ka lumi wela (15-25 ° C)
4. Ua hoʻohui hewa ʻia ka eluent
Manaʻo Manaʻo: E hōʻoia e hoʻohui ʻia ka RNase-Free ddH2O i ka waena o ka membrane o ke kolamu hoʻomaʻemaʻe.
5. Improper leo o anhydrous ethanol ma Buffer viRW2
Manaʻo Manaʻo: E ʻoluʻolu e hahai i nā ʻōlelo aʻoaʻo, e hoʻohui i ka nui kūpono o ka ethanol anhydrous i Buffer viRW2 a hui maikaʻi ma mua o ka hoʻohana ʻana i ka pahu.
6.Improper laʻana hoʻohana.
Manaʻo: 200µl o ka laʻana no 500μl o Buffer viRL.ʻO ka nui o ka nui o ka laʻana e hoʻemi ʻia ka RNA extraction rate.
7. Improper elution volume a i ʻole elution piha ʻole.
Manaʻo: ʻO ka leo eluent o ke kolamu hoʻomaʻemaʻe ʻo 30-50μl;inā ʻaʻole ʻoluʻolu ka hopena elution, pono e hoʻohui i ka RNase-Free ddH pre-heated.2O a hoʻolōʻihi i ka manawa kau ma ka lumi wela, e like me 5-10min
8. He koena ethanol ke kolamu hoʻomaʻemaʻe ma hope o ka holoi ʻana i Buffer viRW2.
Manaʻo Manaʻo: Inā e mau ana ka ethanol ma hope o ka holoi ʻana i Buffer viRW2 a me ka centrifugation pahu pahu ʻole no 2min, hiki ke waiho ʻia ke kolamu hoʻomaʻemaʻe ma ke ana wela no 5min ma hope o ka centrifugation pahu-wai ʻole e wehe loa i ke koena ethanol.
ʻO ka hoʻohaʻahaʻa ʻana o nā molekala RNA i hoʻomaʻemaʻe ʻia
ʻO ka maikaʻi o ka RNA i hoʻomaʻemaʻe ʻia e pili ana i nā mea e like me ka waiho ʻana o ka laʻana, ka haumia RNase, a me ka hana.
Ka nānā 'ana i nā kumu ma'amau:
1. ʻAʻole mālama ʻia nā laʻana i hōʻiliʻili ʻia i ka manawa.
Manaʻo Manaʻo: Inā ʻaʻole hoʻohana ʻia ka hāpana i ka manawa ma hope o ka hōʻiliʻili ʻana, e ʻoluʻolu e mālama iā ia ma -80 ℃ a i ʻole ka nitrogen wai koke.No ka unuhi ʻana i nā molekole RNA, e hoʻāʻo e hoʻohana i nā laʻana i hōʻiliʻili hou ʻia inā hiki.
2. ʻO nā laʻana i hōʻiliʻili ʻia i ka hau a hoʻoheheʻe pinepine ʻia.
Manaʻo Manaʻo: E pale i ka maloʻo a me ka hoʻoheheʻe pinepine ʻana (ʻaʻole i ʻoi aku ma mua o hoʻokahi manawa) i ka wā o ka hōʻiliʻili ʻana a me ka mālama ʻana, inā ʻaʻole e emi ka hua o ka waika nucleic.
3. Ua hoʻokomo ʻia ʻo RNase i loko o ka lumi hana a ʻaʻole i hoʻohana ʻia nā mīkina lima, nā maka, a me nā mea ʻē aʻe.
Manaʻo Manaʻo: E hana maikaʻi ʻia ka hoʻāʻo ʻana o nā moleke RNA i loko o kahi lumi hana RNA ʻokoʻa, a hoʻomaʻemaʻe ʻia ka papa hoʻokolohua ma mua o ka hoʻokolohua.E hoʻohana i nā mīkina lima a me nā maka i ka wā o ka hoʻokolohua e pale aku i ka hōʻino ʻana o RNA ma muli o ka hoʻokomo ʻana o RNase.
4. Ua haumia ka reagent e RNase i ka wā o ka hoʻohana.
Manaʻo: Hoʻololi me ka Viral RNA Isolation Kit hou no nā hoʻokolohua pili.
5. ʻO ka RNase contamination o nā paipu centrifuge, nā ʻōlelo pipette, a me nā mea ʻē aʻe.
Ua hoʻopili ʻia nā molekala RNA i hoʻomaʻemaʻe ʻia i nā hoʻokolohua o lalo
ʻO nā molekala RNA i hoʻomaʻemaʻe ʻia e ke kolamu hoʻomaʻemaʻe e hoʻopili i nā hoʻokolohua i lalo inā he nui nā ion paʻakai a i ʻole nā protein, e like me: reverse transcription, Northern Blot, etc.
1. Aia nā ion paʻakai i koe i loko o nā molekele RNA i hoʻohemo ʻia.
Manaʻo Manaʻo: E hōʻoia ua hoʻohui ʻia ka leo kūpono o ka ethanol anhydrous i Buffer viRW2, a holoi i ke kolamu hoʻomaʻemaʻe ʻelua e like me ka wikiwiki centrifugation pololei ma nā kuhikuhi hana; Inā he mau iona paʻakai i koe, hiki iā ʻoe ke hoʻohui i ka Buffer viRW2 i ke kolamu hoʻomaʻemaʻe, a waiho i ka lumi wela no 5min.A laila e hana i ka centrifugation e wehe i nā ion paʻakai i ka nui loa
2. Aia ke koena ethanol i loko o nā molekele RNA i hoʻokahe ʻia
Manaʻo Manaʻo: i ka manawa e hōʻoia ai ua holoi ʻia nā kolamu hoʻomaʻemaʻe e Buffer viRW2, e hana i ka centrifugation pahu ʻole e like me ka wikiwiki centrifugal ma nā kuhikuhi hana.Inā he ethanol koe, hiki ke waiho ʻia no 5 mau minuke ma ka lumi wela ma hope o ka hoʻoheheʻe ʻia ʻana o ka ethanol no ka wehe ʻana i ke koena ethanol i ka nui loa.
Nā Papa kuhikuhi:
