Foregene DNA Identification System Y Plus ( Non-Extraction)
ʻO ka wehewehe ʻana o ka pahu:
ForegeneHoʻohana ka ʻōnaehana ʻike DNA ʻo Y Plus i ka ʻenehana hōʻailona fluorescent ʻeono kala e hoʻonui ai i ka 20 Y chromosome core loci, 15 loci makemake, 6 loci a me 3 Y indel loci i ka manawa hoʻokahi..
wehewehe
ForegeneHoʻohana ka ʻōnaehana ʻike DNA ʻo Y Plus i ka ʻenehana hōʻailona fluorescent ʻeono kala e hoʻonui ai i ka 20 Y chromosome core loci, 15 loci makemake, 6 loci a me 3 Y indel loci i ka manawa hoʻokahi..
Kiko maʻiʻo
Hoʻomākaukau ʻōnaehana hoʻonui
ʻO kahaku meleo standard(ʻoki ʻole ʻia)ʻōnaehana hoʻonui
| Cnā mea hoʻokūkū | 25 μlʻōnaehana (μl) | 10μl ʻōnaehana (μl) | |
| Master Mix | 4 × Kumu HuiVII | 6.25 | 2.5 |
| 5×Y Hoʻohui Pūmua Hui | 5.0 | 2.0 | |
| wai deionized | 13.75 | 5.5 | |
| Blood lepo | 1.2mm anawaena | 1.2mm anawaena | |
| Total reaction volume | 25 μl | 10 μl | |
ʻIkepili ʻikepili
Pākuʻi 1:Palapala ʻAi Allelic Ladder Type
Pākuʻi 2:DNA type type 9948 typepalapala ʻāina
Waihona Reagent
1. Eʻoluʻolu e mālama ma lalo o -20 ° C (Inā ʻaʻole hoʻohana ʻia no ka manawa pōkole), ma hope o ka loaʻa ʻana o nā pahu hau i ka hau maloʻo a i ʻole nā pahu hau gel.
2. E ʻoluʻolu e mālama i ka mea hana mua 4 × premix VII ma -20 ° C, ma hope o ka lawe ʻia ʻana o ka pahu no ka hoʻohana ʻana, a mālama ʻia nā mea hoʻoponopono hou i koe ma 4 ° C e pale i ka maloʻo a me ka hoʻoheheʻe ʻana.Inā liʻiliʻi ka mea hoʻokahi, ʻōlelo ʻia e mālama ma -20 ° C ma hope o ka ʻai ʻana.
3. E mālama i nā ʻāpana ma hope o ka pane ʻana ma 4 ° C, e pale i ka maloʻo a me ka hoʻoheheʻe pinepine ʻana, a mai hoʻopā i nā reagents ma mua o ka hopena e pale ai i ka haumia.
