ʻO Genomic DNA Micro Kit Micro Genomic DNA Mini Kit
ʻO ka wehewehe ʻana o ka pahu:
E hoʻomaʻemaʻe koke i nā laʻana liʻiliʻi no ka loaʻa ʻana o ka DNA genomic kiʻekiʻe.
◮ʻAʻole hoʻohaumia ʻo RNase:ʻO ka DNA-Only Column i hāʻawi ʻia e ka kit e hiki ai ke wehe i ka RNA mai ka DNA genomic me ka hoʻohui ʻole ʻana i ka RNase i ka wā o ka hoʻokolohua, e pale ana i ka hale hana mai ka haumia ʻana e ka exogenous RNase.
◮wikiwiki wikiwiki:ʻOi aku ka hana o Foregene Protease ma mua o nā protease like, a ʻeli koke i nā ʻāpana kiko;maʻalahi ka hana, a hiki ke hoʻopau ʻia ka hana unuhi DNA genomic i loko o 20-80 mau minuke.
◮ʻoluʻolu:Hana ʻia ka centrifugation ma ka lumi wela, a ʻaʻohe pono no ka 4 ° C haʻahaʻa haʻahaʻa centrifugation a i ʻole ethanol precipitation o DNA.
◮Palekana:ʻAʻole koi ʻia ka hoʻoheheʻe ʻana i nā mea hoʻoheheʻe organik.
◮kūlana kiʻekiʻe:ʻO ka DNA genomic i unuhi ʻia he mau ʻāpana nui, ʻaʻohe RNA, ʻaʻohe RNase, a me ka haʻahaʻa haʻahaʻa haʻahaʻa, hiki ke hoʻokō i nā koi o nā hoʻokolohua like ʻole.
◮Pūnaehana micro-elution:Hiki iā ia ke hoʻonui i ka manaʻo o ka genomic DNA, kahi kūpono no ka ʻike ʻana i lalo a i ʻole ka hoʻokolohua.
wehewehe
Ke hoʻohana nei kēia pahu i kahi DNA-Only Column hiki ke hoʻopaʻa pono i ka DNA, kahi Foregene Protease hou a me kahi ʻōnaehana pale kūʻokoʻa, hiki ke unuhi pono i nā ʻano laʻana olaola like ʻole, e like me ka nui o ke koko, nā cell, a me nā holoholona i loko o 20-80 mau minuke.Hoʻomaʻemaʻe ʻia nā ʻōpala, nā koko maloʻo maloʻo, nā pulupulu lāʻau lapaʻau, nā follicles lauoho, a me nā mea ʻē aʻe, no ka loaʻa ʻana o ka DNA genomic kiʻekiʻe.
Nā kikoʻī
50 Hoʻomākaukau
Nā ʻāpana pahu
| Pale ST1 |
| Hoʻopaʻa ST2* |
| Hoʻopaʻa PW* |
| Hoʻopaʻa WB |
| Hoʻopaʻa EB |
| Foregene Protease |
| DNA-wale wale ke kolamu |
| Nā kuhikuhi |
Nā hiʻohiʻona & pono
-ʻAʻohe RNase contamination: ʻO ka DNA-Only Column i hāʻawi ʻia e ka kit e hiki ai ke wehe i ka RNA mai ka genomic DNA me ka ʻole o ka hoʻohui ʻana i ka RNase i ka wā o ka hoʻokolohua, e pale ana i ka hale hana mai ka haumia ʻana e ka exogenous RNase.
ʻO ka wikiwiki wikiwiki: ʻoi aku ka ʻoi aku o ka ʻoihana Foregene Protease ma mua o nā protease like, a e hoʻoheheʻe koke i nā ʻāpana kiko;maʻalahi ka hana, a hiki ke hoʻopau ʻia ka hana unuhi DNA genomic i loko o 20-80 mau minuke.
-Maikaʻi: Hana ʻia ka centrifugation ma ka lumi wela, a ʻaʻohe pono no ka 4 ° C haʻahaʻa haʻahaʻa centrifugation a i ʻole ethanol precipitation o DNA.
- Palekana: ʻAʻole koi ʻia ka hoʻoheheʻe reagent organik.
-Kiʻekiʻe kiʻekiʻe: ʻO ka DNA genomic i unuhi ʻia he mau ʻāpana nui, ʻaʻohe RNA, ʻaʻohe RNase, a me ka haʻahaʻa haʻahaʻa haʻahaʻa, hiki ke hoʻokō i nā koi o nā hoʻokolohua like ʻole.
- Pūnaehana Micro-elution: Hiki iā ia ke hoʻonui i ka ʻike o ka genomic DNA, kahi kūpono no ka ʻike ʻana i lalo a i ʻole ka hoʻokolohua.
Ke noi pahu
He kūpono ia no ka hoʻomaʻemaʻe ʻana i nā ʻano like ʻole o ka DNA genomic: nā ʻiʻo holoholona hou a i ʻole nā mea maloʻo, ka nui o ke koko holoʻokoʻa a i ʻole anticoagulated, nā ʻāpana koko maloʻo, nā moʻomeheu moʻomeheu, nā ʻiʻo microsection, nā follicles lauoho, nā waha, etc.
Kaʻina hana
Ka mālama ʻana a me ka mālama ʻana
-Hiki ke mālama ʻia kēia kit no 12 mau mahina ma lalo o nā kūlana maloʻo ma ka lumi wela (15-25 ° C);inā pono e mālama ʻia no ka manawa lōʻihi, hiki ke mālama ʻia ma 2-8 ° C.
'Ōlelo Aʻo: Inā mālama ʻia ma kahi wela haʻahaʻa, hiki i ka hāʻina ke hiki i ka ua.Ma mua o ka hoʻohana ʻana, e hōʻoia e waiho i ka hopena i loko o ka pahu i ka lumi wela no kekahi manawa.Inā pono, e hoʻomaʻamaʻa i ka wai ʻauʻau 37 ° C no 10 mau minuke e hoʻoheheʻe i ka wai, a hui ʻia ma mua o ka hoʻohana ʻana.
-Foregene Protease solution he kumu kū hoʻokahi, e hana ana i ka wā e mālama ʻia ai i ka lumi wela no ka manawa lōʻihi (3 mau mahina);E ʻoi aku ka maikaʻi o kāna hana a me ka paʻa ʻana ke mālama ʻia ma 4°C, no laila ua paipai ʻia e mālama ma4°C, e hoʻomanaʻo ʻaʻole e mālama i -20°C .
