Kiko hoʻokaʻawale DNA ʻole kelepona
wehewehe
ʻO DNA me ka cell-free DNA (cfDNA) he ʻāpana o ka cfDNA i hoʻokuʻu ʻia mai nā cell i loko o ke kahe koko e hiki ke lawe i ka ʻike olaola mainā ʻōpū, nā pathogens a me nā fetuses.Hāʻawi ka nānā ʻana o cfDNA i nā māka nui no ka ʻike, ʻike a me ka nānā ʻana i ka holomua o ka maʻision.I kēia manawa, ua hoʻohana nui ʻia kēia ʻenehana i nā ʻano o ka ʻike ʻana i ka mutation tumor, ke alakaʻi ʻana i ka lāʻau lapaʻau a me ka prognosis,ʻike pathogen a me ka nānā ʻana i ka prenatal non-invasive.
Hoʻolālā ʻia ka Foregene Cell-free DNA Isolation Kit no ka hoʻokaʻawale ʻana i ka DNA cell-free kaʻahele (cfDNA) mai ka plasma kanaka, serum, ka wai kino, a i ʻole nā laʻana mimi.Hiki i ka pahu ke hoʻokaʻawale i ka cfDNA i ʻekolu mau ʻanuʻu: lysis/binding, holoi a me ka elution.Hoʻohana ʻo ia i nā peʻa superparamagnetic i hoʻopaʻa ʻia me ka ʻōnaehana lysis/binding kūʻokoʻa e hoʻihoʻi hou i nā ʻakika nucleic e ka hoʻopili hydrogen a me nā ikaika electrostatic, me ka ʻole o ka adsorbing proteins a me nā haumia ʻē aʻe.He kūpono ia no nā keʻena hana hoʻoheheʻe maʻemaʻe kiʻekiʻe ma ka hoʻomākaukau ʻana no ka nānā ʻana i lalo me PCR a i ʻole NGS.
Kiko maʻiʻo a me ka waihona
Papa 1 Foregene Cell-free DNA Isolation Kit
Contents | Reagent | Ka nui(25 T) | Ka nui(50 T) | Ka nui(100 T) | Shoʻomāʻewaʻewa |
Pahu1 | Hoopaa AL | 42 mLx1 hue | 89 mLx1 hue | 189 mLx1 hue | 1 0- 30°C |
Hoʻopaʻa AW1 | 38 mLx1 hue | 83 mLx1 hue | 165 mLx1 hue | 1 0- 30°C | |
Hoʻopaʻa AW2 | 15 mLx1 hue | 33 mLx1 hue | 66 mLx1 hue | 1 0- 30°C | |
Elution | 3 mLx1 hue | 6 mLx1 hue | 12 mLx1 hue | 1 0- 30°C | |
Pahu2 | Nā Moʻi Magnetic A | 3 .2 mLx1 hue | 6 .5 mLx1 hue | 13 mLx1 hue | 2 - 8 ° C |
ʻO ka protein K | 5 mLx1 hue | 10 .5 mLx1 hue | 21 mLx1 hue | 2 - 8 ° C |
Nā memo:
[1] Mai hoʻohui i nā ʻāpana mai nā pūʻulu pahu like ʻole.
[2] Hiki i ka Buffer AL ke hoʻokumu i nā ʻeke, ʻaʻole ia e pili i kāna hana.Inā ʻike ʻia ka ua, e ʻoluʻolu e hoʻokomo i ka ʻōmole reagent i loko o kahi ʻauʻau wai 56 °C no 1 0 - 20 min a hiki i ka hemo ʻana o ka wai.A laila hui pono ma mua o ka hoʻohana ʻana.
[3] Mai hoʻokuʻu i ka Magnetic Beads A.
Kaʻina hana
Nā hiʻohiʻona & pono
-Hoʻopaʻa pono ʻO ke kiʻekiʻe o ka hoʻoheheʻe ʻana o ka DNA, ka maʻemaʻe maikaʻi, ka wehe ʻana i nā mea haumia a me nā mea hoʻopale PCR i lalo;
Hoʻohui Laʻana HikiʻO ka unuhi maʻalahi o ka cfDNA mai 0.5mL a i 4mL nā laʻana me ka nui o ka elution ma lalo o 20uL.
Nā noi like ʻoleHoʻopaʻa ʻia e nā laʻana lapaʻau, i hoʻolālā ʻia no ka maʻi maʻi, ka maʻi a me ka maʻi prenatal me ka biopsy wai
Kākoʻo ākea o Automated Platform Hoʻohālikelike me nā kaʻina hana hoʻoheheʻe automated, kākoʻo iā Tecan, Hamilton, PE a me nā keʻena hana hoʻoneʻe nucleic acid.