ʻO ka mea kūʻai gula ʻo Kina no Kina ka Laʻana manuahi 32t 64t 96t DNA Rna Nucleic Acid Isolation Reagent Kit Mai ka Mea Hana Uni-Medica me CE a me FDA.
ʻO ka wehewehe ʻana o ka pahu:
ʻAʻole pono e hopohopo e pili ana i ka degradation RNA.ʻO ka ʻōnaehana holoʻokoʻa he RNase-Free
Hoʻopau maikaʻi i ka DNA me ka hoʻohana ʻana i ke kolamu hoʻomaʻemaʻe DNA
Wehe i ka DNA me ka hoʻohui ʻole i ka DNase
Maʻalahi—ua pau nā hana a pau i ka wela lumi
Hiki ke hoʻopau i ka hana i loko o 30 mau minuke
Palekana—ʻaʻole hoʻohana ʻia ka mea hoʻoheheʻe organik
Maʻemaʻe kiʻekiʻe—OD260/280≈1.8-2.1
Ma muli o ka hui maikaʻi loa, nā ʻano mea kūʻai kiʻekiʻe o ka laulā, nā uku hoʻokūkū a me ka hoʻopuka maikaʻi ʻana, hauʻoli mākou i kahi moʻolelo maikaʻi loa i waena o kā mākou mea kūʻai.He hui ikaika mākou me ka mākeke ākea no China Gold Supplier for China Free Sample 32t 64t 96t DNA Rna Nucleic Acid Isolation Reagent Kit Mai Original Manufacturer Uni-Medica me CE a me FDA.
Ma muli o ka hui maikaʻi loa, nā ʻano mea kūʻai kiʻekiʻe o ka laulā, nā uku hoʻokūkū a me ka hoʻopuka maikaʻi ʻana, hauʻoli mākou i kahi moʻolelo maikaʻi loa i waena o kā mākou mea kūʻai.He hui ikaika mākou me ka mākeke ākea noKina Nucleic Acid Isolation Reagent, Nucleic Acid Extraction Reagent, Me nā mea kūʻai kiʻekiʻe loa, ka lawelawe ma hope o ke kūʻai aku a me ke kulekele hōʻoia, lanakila mākou i ka hilinaʻi mai nā hoa ʻē aʻe he nui, nui nā manaʻo maikaʻi i ʻike i ka ulu ʻana o kā mākou hale hana.Me ka hilinaʻi piha a me ka ikaika, hoʻokipa i nā mea kūʻai aku e hoʻopili a kipa mai iā mākou no ka pilina e hiki mai ana.
Nā wehewehe kits
50 Mākaukau, 200 Mākaukau
Ke hoʻohana nei kēia kit i ka kolamu spin a me ka formula i hoʻomohala ʻia e kā mākou hui, hiki ke unuhi i ka RNA holoʻokoʻa kiʻekiʻe-maʻemaʻe a kiʻekiʻe loa mai nā ʻano holoholona like ʻole me ka hana kiʻekiʻe.Hiki i ke kolamu RNA-wale ke hoʻopaʻa maikaʻi i ka RNA a hiki ke hana ʻia i ka manawa like me kahi ʻano ʻokoʻa He nui nā laʻana.
ʻO ka pūnaewele holoʻokoʻa he RNase-Free, iʻole e hoʻohaʻahaʻaʻia ka RNA i unuhiʻia;Buffer RW1, Buffer RW2 ʻōnaehana holoi holoi, no laila ʻo ka RNA i loaʻa ʻole i ka protein, DNA, ion, a me ka pollution hui.
Nā ʻāpana pahu:
| Hoʻopaʻa RL1 , Hoʻopaʻa RL2 |
| Hoʻopaʻa RW1, Hoʻopaʻa RW2 |
| RNase-Free ddH2O, Kona Hoʻomaʻemaʻe DNA |
| RNA-Kola wale nō |
Nā hiʻohiʻona & pono
■ ʻAʻole pono e hopohopo no ka hoʻohaʻahaʻa ʻana o RNA;ʻo RNase-Free ka ʻōnaehana holoʻokoʻa
■ Hoʻopau maikaʻi i ka DNA-hoʻohana i ka DNA-Cleaning Column
■ Wehe i ka DNA me ka hookomo ole i ka DNase
■ Maalahi-ua pau na hana a pau ma ka wela lumi
■ Hiki ke hoʻopau i ka hana wikiwiki i 30 mau minuke
■ Palekana-ʻaʻole pono ka mea hoʻoheheʻe organik
■ Maʻemaʻe kiʻekiʻe -OD260/280≈1.8-2.1
Ke noi pahu
He kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka RNA holoʻokoʻa mai nā ʻano ʻiʻo holoholona hou a hauʻoli paha a i ʻole nā pūnaewele moʻomeheu.
Nā palena huahana
■ Nā noi i lalo: ka hoʻohui ʻana o ka cDNA mua, RT-PCR, molecular cloning, Northern Blot, etc.
■ Nā laʻana: nā ʻiʻo holoholona, nā pūnaewele moʻomeheu
■ Ka Papa Hana: Tissues 10-20mg, Cells(2-5)×106
■ Ka hiki ke hoʻopaʻa DNA kiʻekiʻe o ke kolamu hoʻomaʻemaʻe: 80 μg
■ Ka nui elution: 50-200 μl
Ka holo hana
Diagram
ʻO ka Animal Total RNA Isolation Kit i mālama ʻia he 20mg
Nā laʻana ʻiole hou, e lawe i ka 5% i hoʻomaʻemaʻe ʻia ka RNA 1% agar
ʻO ka electrophoresis glycogel
1: Spleen 2: Kidney
3: Ake 4: Puʻuwai
Ka mālama ʻana a me ka mālama ʻana
Hiki ke mālama ʻia ka pahu no 24 mau mahina ma ka lumi wela (15–25 ℃) a i ʻole 2–8 ℃ no ka manawa lōʻihi.Hiki ke mālama ʻia ʻo Buffer RL1 ma 4 ℃ no 1 mahina ma hope o ka hoʻohui ʻana i ka β- mercaptoethanol (koho).He hui ikaika mākou me ka mākeke ākea no China Gold Supplier for China Free Sample 32t 64t 96t DNA Rna Nucleic Acid Isolation Reagent Kit Mai Original Manufacturer Uni-Medica me CE a me FDA.
Kina mea kuai gula noKina Nucleic Acid Isolation Reagent, Nucleic Acid Extraction Reagent, Me nā mea kūʻai kiʻekiʻe loa, ka lawelawe ma hope o ke kūʻai aku a me ke kulekele hōʻoia, lanakila mākou i ka hilinaʻi mai nā hoa ʻē aʻe he nui, nui nā manaʻo maikaʻi i ʻike i ka ulu ʻana o kā mākou hale hana.Me ka hilinaʻi piha a me ka ikaika, hoʻokipa i nā mea kūʻai aku e hoʻopili a kipa mai iā mākou no ka pilina e hiki mai ana.
ʻAʻole lawe ʻia ka RNA a i ʻole nā hua RNA
Nui nā ʻano like ʻole e pili ana i ka pono o ka hoʻihoʻi hou ʻana, e like me: kiko kiko RNA maʻiʻo, ke ʻano o ka hana, ka nui elution, etc.
1. Hana ʻia ka ʻauʻau hau a i ʻole cryogenic (4 °C) centrifugation i ka wā o ka hana.
Manaʻo: E hana ma ka lumi wela (15-25 ° C) i ke kaʻina holoʻokoʻa, mai ʻauʻau hau a centrifuge i nā haʻahaʻa haʻahaʻa.
2. Ka mālama pono ʻole ʻana i ka laʻana a i ʻole ka nui o ka manawa e mālama ai i ka laʻana.
Manaʻo Manaʻo: E kūʻai i nā laʻana ma -80 °C a i ʻole i loko o ka hau hau i loko o ka wai hau a pale i ka hoʻohana pinepine ʻana i ka hau hau;e ho'āʻo e hoʻohana i ka ʻiʻo hou a i ʻole nā pūnaewele moʻomeheu no ka unuhi ʻana i ka RNA.
3. lawa ole la'ana lysis.
Manaʻo Manaʻo: I ka wā e hoʻohui ai i ka ʻiʻo, e hōʻoia i ka hoʻomaʻemaʻe ʻia ʻana o ka ʻiʻo a ua hoʻokaʻawale ʻia nā mea kikoo e wehewehe i ka hoʻokuʻu ʻana o RNA.
4. ʻAʻole i hoʻohui pono ʻia ka eluent.
Manaʻo: E hōʻoia i ka RNase-Free ddH2Hoʻohui ʻia ʻo O i kahi dropwise i ka waena o ka membrane kolamu hoʻomaʻemaʻe.
5. ʻAʻole i hoʻohui ʻia ka leo kūpono o ka ethanol piha i ka Buffer RL2 a i ʻole Buffer RW2.
Manaʻo Manaʻo: E hahai i nā kuhikuhi, e hoʻohui i ka leo kūpono o ka ethanol piha i ka Buffer RL2 a me Buffer RW2 a hui maikaʻi ma mua o ka hoʻohana ʻana i ka pahu.
6. ʻAʻole kūpono ke ʻano o ka laʻana.
Manaʻo: E hoʻohana i ka 10-20 mg o ka ʻiʻo a i ʻole (1-5) × 106nā pūnaewele no 500 μl buffer RL1, no ka mea, hiki i ka hoʻohana ʻana i ka ʻiʻo nui ke hopena i ka hōʻemi ʻana i ka RNA extraction.
7. Ka nui elution kūpono ʻole a i ʻole ka elution piha ʻole.
Manaʻo: ʻO ka nui elution o ke kolamu hoʻomaʻemaʻe ʻo 50-200 μl;inā ʻaʻole ʻoluʻolu ka hopena elution, pono ia e hoʻolōʻihi i ka manawa kau wela o ka lumi ma hope o ka hoʻohui ʻana i ka RNase-Free ddH preheated.2ʻO, e laʻa no 5-10 min.
8. He koena ethanol ka kolamu hoʻomaʻemaʻe ma hope o ka holoi ʻana o Buffer RW2.
Manaʻo Manaʻo: Inā loaʻa ke koena ethanol ma hope o ka holoi ʻana o Buffer RW2, ʻo ka centrifugation paipu ʻole no 1min, hiki ke hoʻonui ʻia ka manawa no ka hana centrifugation pahu ʻole i 2min, a i ʻole ke kolamu hoʻomaʻemaʻe hiki ke kau ʻia ma ka lumi wela no 5 min e wehe pono i ke koena ethanol.
Hoʻohaʻahaʻa ʻia ka RNA hoʻomaʻemaʻe
ʻO ka maikaʻi o ka RNA i hoʻomaʻemaʻe ʻia e pili ana i nā mea e like me ka mālama ʻana i ka laʻana, RNase contamination, a me ka manipulation, etc.
1. ʻAʻole mālama ʻia nā ʻāpana kiko i ka manawa.
Manaʻo Manaʻo: Inā ʻaʻole hoʻohana ʻia nā laʻana a i ʻole nā pūnaewele i ka manawa kūpono ma hope o ka hōʻiliʻili ʻana, e mālama koke i ka cryopreserve ma -80 °C a i ʻole ka wai nitrogen.No ka unuhi ʻana i ka RNA, e hoʻohana i kahi ʻiʻo i lawe ʻia a i ʻole ka laʻana cell i ka wā hiki.
2. ʻO ka hoʻoheheʻe hou ʻia ʻana o nā laʻana kiko.
Manaʻo Manaʻo: I ka wā e mālama ai i nā laʻana ʻiʻo, ʻoi aku ka maikaʻi e ʻoki ʻia i mau ʻāpana liʻiliʻi no ka mālama ʻana, a e wehe i kekahi o nā ʻāpana i ka wā e hoʻohana ai ia mau mea e pale aku ai i ka hoʻoheheʻe pinepine ʻana o ka hāpana a me ka hōʻino ʻana o RNA.
3. Hoʻokomo ʻia ʻo RNase a ʻaʻole hoʻohana i nā mīkina lima hoʻolele, masks, etc. i ka wā o ka hana.
Manaʻo Manaʻo: Hana maikaʻi ʻia nā hoʻokolohua RNA extraction ma nā keʻena hoʻoponopono RNA kaʻawale a holoi ʻia ka papaʻaina ma mua o ka hoʻokolohua.
E hoʻohana i nā mīkina lima a me nā maka i ka wā o ka hoʻokolohua e hōʻemi i ka hōʻemi ʻana o RNA ma muli o ka hoʻokomo ʻana o RNase.
4. Ho'ohaumia 'ia nā reagents me RNase i ka wā e ho'ohana ai.
Manaʻo Manaʻo: E hoʻololi me kahi Animal Total RNA Isolation Kit no nā hoʻokolohua pili.
5. ʻO nā paipu centrifuge, nā ʻōlelo aʻoaʻo, a me nā mea ʻē aʻe i hoʻohana ʻia i ka RNA manipulation ua haumia me RNase.
Manaʻo Manaʻo: E hōʻoia i ka RNase-Free nā paipu centrifuge, nā ʻōlelo aʻoaʻo, nā pipette, a me nā mea ʻē aʻe i hoʻohana ʻia i ka unuhi RNA.
Hoʻopili ka RNA i hoʻomaʻemaʻe ʻia i nā hoʻokolohua ma lalo
Hoʻomaʻemaʻe ʻia ʻo RNA e ke kolamu hoʻomaʻemaʻe, inā ʻoi aku ka nui o nā ion paʻakai, ʻoi aku ka nui o ka protein ma lalo o ka hoʻokolohua, e like me: reverse transcription, Northern Blot et al.
1. He koena iona paakai ko ka RNA i hooleiia.
Manaʻo Manaʻo: E hōʻoia ua hoʻohui ʻia ka nui kūpono o ka ethanol i ka Buffer RW2 a hana i 2 holoi kolamu hoʻomaʻemaʻe ma ka wikiwiki centrifugal i hōʻike ʻia no ka hana;inā loaʻa kekahi koena ion paʻakai, waiho i ke kolamu hoʻomaʻemaʻe iā Buffer RW2 no 5 min ma ka lumi wela a hana centrifugation e hoʻonui i ka wehe ʻana o ka paʻakai.
2. Ke koena ethanol i loko o ka RNA i hoʻoheheʻe ʻia.
Manaʻo Manaʻo: E hōʻoia ma hope o ka holoi ʻana i ka RW2, e hoʻokō i ka hana centrifugation paipu ʻole ma ka wikiwiki centrifugation i hōʻike ʻia no ka hana, e hoʻonui i ka manawa o ka hana centrifugation paipu ʻole i 2 min inā he koena ethanol, a i ʻole waiho ʻia ma ka lumi wela no 5 min ma hope o ka centrifugation pahu hakahaka e hoʻonui i ka wehe ʻana o ke koena ethanol.
