• facebook
  • linkedin
  • youtube
English
ʻaoʻao_banner

ʻO EndoFree Maxi Plasmid Kit (Kolana Milo)

ʻO ka wehewehe ʻana o ka pahu:

ʻO ke kaʻina hana unuhi holoʻokoʻa he 1 hola wale nō, e hōʻoia ana i ka hana maʻalahi a wikiwiki.

ikaika mua


Huahana Huahana

Huahana Huahana

wehewehe

Hoʻohana ka EndoFree Maxi Plasmid Kit i kahi ʻenehana adsorption silica membrane kūʻokoʻa e hoʻopaʻa pono ai i ka DNA plasmid.Ma ka hoʻohui ʻana i ka Endotoxin Removal Buffer P4 a me EndoFree Maxi Filtration Columns, endotoxin, proteins a me nā mea haumia ʻē aʻe hiki ke wehe maikaʻi ʻia.ʻO ke kaʻina hana unuhi holoʻokoʻa he 1 hola wale nō, e hōʻoia ana i ka hana maʻalahi a wikiwiki.

ʻO ka nui o ka bacteria i manaʻo ʻia no kēlā me kēia hana: No ka plasmid kope kiʻekiʻe, pono e hoʻohana i 100 ml o ka media moʻomeheu bacteria, a ʻo ka hua mai ia mea ma kahi o 500-1500 μg.No ka plasmid kope haʻahaʻa, ʻōlelo ʻia ka 200 ml bacteria moʻomeheu media e hana ma kahi o 200-600 μg plasmids.

Nā ʻāpana pahu

Huahana Nā ʻāpana

D909-E(10 hoʻomākaukau)
Hoʻopaʻa BL 30 ml
Palena P1 100 ml 
Palena P2 100 ml 
Palena P4 100 ml
Hoʻopaʻa DW2 44 ml
Hoʻopaʻa EB 30 ml
RNase A(10 mg/ml) 1 ml
DNAPure Maxi Spin Colums 10 pcs
ʻO FinePure Maxi Filter Syringe 10 pcs
50 ml mau paipu ohi 10 pcs

Ka ʻike ʻana o ka Plasmid DNA Concentration a me ka Maʻemaʻe

Hiki ke ʻike ʻia ka maʻemaʻe a me ka noʻonoʻo o ka DNA plasmid i unuhi ʻia e ka electrophoresis gel agarose a me kahi spectrophotometer ultraviolet.ʻO ka waiwai OD260 o 1 pili i ka 50 μg/ml o ka DNA stranded pālua.ʻO ka waiwai OD260/OD280 o ka DNA plasmid i hoʻomaʻemaʻe ʻia ma ka laulā o 1.7-1.9.Hiki ke hoʻopili pololei ʻia ka DNA plasmid i hoʻomaʻemaʻe ʻia i nā hoʻokolohua me nā koi maʻemaʻe kiʻekiʻe, e like me ka neʻe ʻana o ke kelepona a i ʻole nā ​​hoʻokolohua vivo.

Ke noi pahu

Hiki ke hoʻohana ʻia ka plasmid DNA i unuhi ʻia e kēia kit i nā hana maʻamau, e like me ka enzymatic digestion, PCR, sequencing, ligation, hoʻololi a me ka hoʻololi ʻana no nā ʻano cell like ʻole.

Ka mālama ʻana a me ka mālama ʻana

Hiki ke mālama ʻia ʻo EndoFree Maxi Plasmid Kit ma ka lumi wela (15-25°C) ma lalo o nā kūlana maloʻo a hiki i 12 mahina.E hoʻohui i ka RNase A i ka Buffer P1 ma mua o ka hoʻohana mua ʻana, hui maikaʻi a mālama i ka 2-8°C.Hiki ke mālama pono ʻia ka hui ʻana ma mua o 6 mau mahina.

  • Mua:
  • Aʻe:
    • E kākau i kāu leka ma aneʻi a hoʻouna mai iā mākou

    PiliNā huahana

    E kaomi i ke komo no ka huli ʻana a i ʻole ESC e pani