ʻO OEM Factory no Kina Virus Magnetic Bead Method Rna-DNA Nucleic Acid Sample Extraction Purification Tube Kit
ʻO ka loaʻa ʻana o ka ʻoluʻolu o nā mea kūʻai aku ke kumu o kā mākou hui me ka pau ʻole.E hana mākou i nā hana maikaʻi e hana i nā mea kūʻai hou a maikaʻi loa, e hoʻokō i kāu mau koi kūʻokoʻa a hāʻawi iā ʻoe i ke kūʻai mua ʻana, ma ke kūʻai aku a ma hope o ke kūʻai aku ʻana no ka OEM Factory no China Virus Magnetic Bead Method Rna-DNANucleic AcidSample Extraction Purification Tube Kit, Ke kū nei i kēia lā a nānā i ka wā e hiki mai ana, mahalo mākou i nā mea kūʻai aku ma ka honua holoʻokoʻa e hui pū me mākou.
ʻO ka loaʻa ʻana o ka ʻoluʻolu o nā mea kūʻai aku ke kumu o kā mākou hui me ka pau ʻole.E hana mākou i nā hana kupanaha e hana i nā mea kūʻai hou a kiʻekiʻe loa, e hoʻokō i kāu mau koi kūʻokoʻa a hāʻawi iā ʻoe i nā lawelawe pre-sale, ma ke kūʻai aku a ma hope o ke kūʻai aku.ʻO ka pahu hoʻoheheʻe Kina, Nucleic Acid, Me ke kākoʻo ʻenehana maikaʻi loa, ua hoʻohālikelike mākou i kā mākou pūnaewele no ka ʻike mea hoʻohana maikaʻi loa a mālama i kou maʻalahi o ke kūʻai.hōʻoia mākou e loaʻa iā ʻoe ka mea maikaʻi loa ma kou ʻīpuka, i ka manawa pōkole loa a me ke kōkua ʻana o kā mākou mau hoa logistical maikaʻi ʻo DHL a me UPS.Ke hoʻohiki nei mākou i ka maikaʻi, e ola ana ma ka motto o ka hoʻohiki wale i ka mea hiki iā mākou ke hāʻawi.
Nā wehewehe kits
50 Mākaukau, 200 Mākaukau
Ke hoʻohana nei kēia kit i ka kolamu spin a me ka formula i hoʻomohala ʻia e kā mākou hui, hiki ke unuhi i ka RNA holoʻokoʻa kiʻekiʻe-maʻemaʻe a kiʻekiʻe loa mai nā ʻano holoholona like ʻole me ka hana kiʻekiʻe.Hiki i ke kolamu RNA-wale ke hoʻopaʻa maikaʻi i ka RNA a hiki ke hana ʻia i ka manawa like me kahi ʻano ʻokoʻa He nui nā laʻana.
ʻO ka pūnaewele holoʻokoʻa he RNase-Free, iʻole e hoʻohaʻahaʻaʻia ka RNA i unuhiʻia;Buffer RW1, Buffer RW2 ʻōnaehana holoi holoi, no laila ʻo ka RNA i loaʻa ʻole i ka protein, DNA, ion, a me ka pollution hui.
Nā ʻāpana pahu:
Hoʻopaʻa RL1 , Hoʻopaʻa RL2 |
Hoʻopaʻa RW1, Hoʻopaʻa RW2 |
RNase-Free ddH2O, Kona Hoʻomaʻemaʻe DNA |
RNA-Kola wale nō |
Nā hiʻohiʻona & pono
■ ʻAʻole pono e hopohopo no ka hoʻohaʻahaʻa ʻana o RNA;ʻo RNase-Free ka ʻōnaehana holoʻokoʻa
■ Hoʻopau maikaʻi i ka DNA-hoʻohana i ka DNA-Cleaning Column
■ Wehe i ka DNA me ka hookomo ole i ka DNase
■ Maalahi-ua pau na hana a pau ma ka wela lumi
■ Hiki ke hoʻopau i ka hana wikiwiki i 30 mau minuke
■ Palekana-ʻaʻole pono ka mea hoʻoheheʻe organik
■ Maʻemaʻe kiʻekiʻe -OD260/280≈1.8-2.1
Ke noi pahu
He kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka RNA holoʻokoʻa mai nā ʻano ʻiʻo holoholona hou a hauʻoli paha a i ʻole nā pūnaewele moʻomeheu.
Nā palena huahana
■ Nā noi i lalo: ka hoʻohui ʻana o ka cDNA mua, RT-PCR, molecular cloning, Northern Blot, etc.
■ Nā laʻana: nā ʻiʻo holoholona, nā pūnaewele moʻomeheu
■ Ka Papa Hana: Tissues 10-20mg, Cells(2-5)×106
■ Ka hiki ke hoʻopaʻa DNA kiʻekiʻe o ke kolamu hoʻomaʻemaʻe: 80 μg
■ Ka nui elution: 50-200 μl
Ka holo hana
Diagram
ʻO ka Animal Total RNA Isolation Kit i mālama ʻia he 20mg
Nā laʻana ʻiole hou, e lawe i ka 5% i hoʻomaʻemaʻe ʻia ka RNA 1% agar
ʻO ka electrophoresis glycogel
1: Spleen 2: Kidney
3: Ake 4: Puuwai
Ka mālama ʻana a me ka mālama ʻana
Hiki ke mālama ʻia ka pahu no 24 mau mahina ma ka lumi wela (15–25 ℃) a i ʻole 2–8 ℃ no ka manawa lōʻihi.Hiki ke mālama ʻia ʻo Buffer RL1 ma 4 ℃ no 1 mahina ma hope o ka hoʻohui ʻana i ka β- mercaptoethanol (koho).E hana mākou i nā hana maikaʻi e hana i nā mea kūʻai hou a maikaʻi loa, e hoʻokō i kāu mau koi kūʻokoʻa a hāʻawi iā ʻoe i ke kūʻai mua ʻana, ma ke kūʻai aku a ma hope o ke kūʻai aku ʻana no ka OEM Factory no China Virus Magnetic Bead Method Rna-DNANucleic AcidSample Extraction Purification Tube Kit, Ke kū nei i kēia lā a nānā i ka wā e hiki mai ana, mahalo mākou i nā mea kūʻai aku ma ka honua holoʻokoʻa e hui pū me mākou.
OEM Factory noʻO ka pahu hoʻoheheʻe Kina, Nucleic Acid, Me ke kākoʻo ʻenehana maikaʻi loa, ua hoʻohālikelike mākou i kā mākou pūnaewele no ka ʻike mea hoʻohana maikaʻi loa a mālama i ka maʻalahi o kāu kūʻai.hōʻoia mākou e loaʻa iā ʻoe ka mea maikaʻi loa ma kou ʻīpuka, i ka manawa pōkole loa a me ke kōkua ʻana o kā mākou mau hoa logistical maikaʻi ʻo DHL a me UPS.Ke hoʻohiki nei mākou i ka maikaʻi, e ola ana ma ka motto o ka hoʻohiki wale i ka mea hiki iā mākou ke hāʻawi.
ʻAʻole lawe ʻia ka RNA a i ʻole nā hua RNA
Nui nā ʻano like ʻole e pili ana i ka pono o ka hoʻihoʻi hou ʻana, e like me: kiko kiko RNA maʻiʻo, ke ʻano o ka hana, ka nui elution, etc.
1. Hana ʻia ka ʻauʻau hau a i ʻole cryogenic (4 °C) centrifugation i ka wā o ka hana.
Manaʻo: E hana ma ka lumi wela (15-25 ° C) i ke kaʻina holoʻokoʻa, mai ʻauʻau hau a centrifuge i nā haʻahaʻa haʻahaʻa.
2. Ka mālama pono ʻole ʻana i ka laʻana a i ʻole ka nui o ka manawa e mālama ai i ka laʻana.
Manaʻo Manaʻo: E kūʻai i nā laʻana ma -80 °C a i ʻole i loko o ka hau hau i loko o ka wai hau a pale i ka hoʻohana pinepine ʻana i ka hau hau;e ho'āʻo e hoʻohana i ka ʻiʻo hou a i ʻole nā pūnaewele moʻomeheu no ka unuhi ʻana i ka RNA.
3. lawa ole la'ana lysis.
Manaʻo Manaʻo: I ka wā e hoʻohui ai i ka ʻiʻo, e hōʻoia i ka hoʻomaʻemaʻe ʻia ʻana o ka ʻiʻo a ua hoʻokaʻawale ʻia nā mea kikoo e wehewehe i ka hoʻokuʻu ʻana o RNA.
4. ʻAʻole i hoʻohui pono ʻia ka eluent.
Manaʻo: E hōʻoia i ka RNase-Free ddH2Hoʻohui ʻia ʻo O i kahi dropwise i ka waena o ka membrane kolamu hoʻomaʻemaʻe.
5. ʻAʻole i hoʻohui ʻia ka leo kūpono o ka ethanol piha i ka Buffer RL2 a i ʻole Buffer RW2.
Manaʻo Manaʻo: E hahai i nā kuhikuhi, e hoʻohui i ka leo kūpono o ka ethanol piha i ka Buffer RL2 a me Buffer RW2 a hui maikaʻi ma mua o ka hoʻohana ʻana i ka pahu.
6. ʻAʻole kūpono ke ʻano o ka laʻana.
Manaʻo: E hoʻohana i ka 10-20 mg o ka ʻiʻo a i ʻole (1-5) × 106nā pūnaewele no 500 μl buffer RL1, no ka mea, hiki i ka hoʻohana ʻana i ka ʻiʻo nui ke hopena i ka hōʻemi ʻana i ka RNA extraction.
7. Ka nui elution kūpono ʻole a i ʻole ka elution piha ʻole.
Manaʻo: ʻO ka nui elution o ke kolamu hoʻomaʻemaʻe ʻo 50-200 μl;inā ʻaʻole ʻoluʻolu ka hopena elution, pono ia e hoʻolōʻihi i ka manawa kau wela o ka lumi ma hope o ka hoʻohui ʻana i ka RNase-Free ddH preheated.2ʻO, e laʻa no 5-10 min.
8. He koena ethanol ka kolamu hoʻomaʻemaʻe ma hope o ka holoi ʻana o Buffer RW2.
Manaʻo Manaʻo: Inā loaʻa ke koena ethanol ma hope o ka holoi ʻana o Buffer RW2, ʻo ka centrifugation paipu ʻole no 1min, hiki ke hoʻonui ʻia ka manawa no ka hana centrifugation pahu ʻole i 2min, a i ʻole ke kolamu hoʻomaʻemaʻe hiki ke kau ʻia ma ka lumi wela no 5 min e wehe pono i ke koena ethanol.
Hoʻohaʻahaʻa ʻia ka RNA hoʻomaʻemaʻe
ʻO ka maikaʻi o ka RNA i hoʻomaʻemaʻe ʻia e pili ana i nā mea e like me ka mālama ʻana i ka laʻana, RNase contamination, a me ka manipulation, etc.
1. ʻAʻole mālama ʻia nā ʻāpana kiko i ka manawa.
Manaʻo Manaʻo: Inā ʻaʻole hoʻohana ʻia nā laʻana a i ʻole nā pūnaewele i ka manawa kūpono ma hope o ka hōʻiliʻili ʻana, e mālama koke i ka cryopreserve ma -80 °C a i ʻole ka wai nitrogen.No ka unuhi ʻana i ka RNA, e hoʻohana i kahi ʻiʻo i lawe ʻia a i ʻole ka laʻana cell i ka wā hiki.
2. ʻO ka hoʻoheheʻe hou ʻia ʻana o nā laʻana kiko.
Manaʻo Manaʻo: I ka wā e mālama ai i nā laʻana ʻiʻo, ʻoi aku ka maikaʻi e ʻoki ʻia i mau ʻāpana liʻiliʻi no ka mālama ʻana, a e wehe i kekahi o nā ʻāpana i ka wā e hoʻohana ai ia mau mea e pale aku ai i ka hoʻoheheʻe pinepine ʻana o ka hāpana a me ka hōʻino ʻana o RNA.
3. Hoʻokomo ʻia ʻo RNase a ʻaʻole hoʻohana i nā mīkina lima hoʻolele, masks, etc. i ka wā o ka hana.
Manaʻo Manaʻo: Hana maikaʻi ʻia nā hoʻokolohua RNA extraction ma nā keʻena hoʻoponopono RNA kaʻawale a holoi ʻia ka papaʻaina ma mua o ka hoʻokolohua.
E hoʻohana i nā mīkina lima a me nā maka i ka wā o ka hoʻokolohua e hōʻemi i ka hōʻemi ʻana o RNA ma muli o ka hoʻokomo ʻana o RNase.
4. Ho'ohaumia 'ia nā reagents me RNase i ka wā e ho'ohana ai.
Manaʻo Manaʻo: E hoʻololi me kahi Animal Total RNA Isolation Kit no nā hoʻokolohua pili.
5. ʻO nā paipu centrifuge, nā ʻōlelo aʻoaʻo, a me nā mea ʻē aʻe i hoʻohana ʻia i ka RNA manipulation ua haumia me RNase.
Manaʻo Manaʻo: E hōʻoia i ka RNase-Free nā paipu centrifuge, nā ʻōlelo aʻoaʻo, nā pipette, a me nā mea ʻē aʻe i hoʻohana ʻia i ka unuhi RNA.
Hoʻopili ka RNA i hoʻomaʻemaʻe ʻia i nā hoʻokolohua ma lalo
Hoʻomaʻemaʻe ʻia ʻo RNA e ke kolamu hoʻomaʻemaʻe, inā ʻoi aku ka nui o nā ion paʻakai, ʻoi aku ka nui o ka protein ma lalo o ka hoʻokolohua, e like me: reverse transcription, Northern Blot et al.
1. He koena iona paakai ko ka RNA i hooleiia.
Manaʻo Manaʻo: E hōʻoia ua hoʻohui ʻia ka nui kūpono o ka ethanol i ka Buffer RW2 a hana i 2 holoi kolamu hoʻomaʻemaʻe ma ka wikiwiki centrifugal i hōʻike ʻia no ka hana;inā loaʻa kekahi koena ion paʻakai, waiho i ke kolamu hoʻomaʻemaʻe iā Buffer RW2 no 5 min ma ka lumi wela a hana centrifugation e hoʻonui i ka wehe ʻana o ka paʻakai.
2. Ke koena ethanol i loko o ka RNA i hoʻoheheʻe ʻia.
Manaʻo Manaʻo: E hōʻoia ma hope o ka holoi ʻana i ka RW2, e hoʻokō i ka hana centrifugation paipu ʻole ma ka wikiwiki centrifugation i hōʻike ʻia no ka hana, e hoʻonui i ka manawa o ka hana centrifugation paipu ʻole i 2 min inā he koena ethanol, a i ʻole waiho ʻia ma ka lumi wela no 5 min ma hope o ka centrifugation pahu hakahaka e hoʻonui i ka wehe ʻana o ke koena ethanol.