ʻO Kina SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method) Mea hana a mea hoʻolako |Foregene

ʻO SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)

ʻO ka wehewehe ʻana o ka pahu:

◮ PCR pololei:ʻAʻohe pono no ka pahu nucleic acid extaction kit a me System, hoʻopau i 96 specimen i 1 hola a i ʻole.
◮ Pono nā mea hana haʻahaʻa a me ka hoʻohana maʻalahi loa:Pono wale ka ʻōnaehana PCR Real-Time.
◮ LoD a me ka ʻike kiʻekiʻe:Hiki i ka ʻike ʻike ke piʻi i ka pae haʻahaʻa e like me 500 kope/ml.

E hoʻouna i ka leka uila iā mākou Hoʻoiho ma ke ʻano he PDF

Huahana Huahana

Hoohana Manao

Ke hoʻohana nei kēia pahu i ka ʻenehana RT PCR Real-time (rRT-PCR) no ka ʻike qualitative o SARS-CoV-2 nucleic acids i nā nasopharyngeal a i ʻole oropharyngeal swab samples.

Kumu o ka hoao ana

Hoʻolālā ʻia kēia ʻano kumu mua a me ka ʻimi noiʻi no ka ʻike ʻana i nā kaʻina i mālama ʻia o SARS-CoV-2 (ORF1ab gene, N gene a me E gene).Aia i loko o ka pahu kahi Mana Kūloko i hoʻohana ʻia e loiloi i ka maikaʻi o nā mea hoʻohālike.Hoʻohana ka ʻenehana PCR Real-Time i ka hopena polymerase chain reaction (PCR) no ka hoʻonui ʻana i nā kaʻina kikoʻī kikoʻī a me nā ʻimi kikoʻī kikoʻī no ka ʻike ʻana i ka RNA i hoʻonui ʻia.Hoʻopaʻa inoa ʻia nā ʻimi noiʻi me ka mea hoʻolaha fluorescent a me nā mea hoʻonaninani kinai.

Nā kikoʻī wikiwiki

◮Nā kikoʻī:48 Rxns/kit, 96 Rxns/kit

◮ ʻano kikoʻī:96.72%

ʻIkepili:97.92%

◮ʻAno hoʻohālike pili

Nasopharyngeal Swab or Oropharyngeal Swab

◮Mea hana pili

ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S

Nā ʻāpana

ʻAʻole.	ʻāpana	Ka nui				Mea nui
ʻAʻole.	ʻāpana	48 Rxns		96 Rxns		Mea nui
1	Hoʻokuʻu ʻia ka waika nucleicluna	1.5 mL / paipu	1 paipu	1.5 mL / paipu	1 paipu	Mea hoʻokuʻu
2	RNA pale	27 μL / paipu	1 paipu	53 μL / paipu	1 paipu	ʻO ka mea hoʻopaneʻe RNase
3	SARS-CoV-2hoʻonā hopena	800 μL / paipu	1 paipu	1600 μL / paipu	1 paipu	Primer, probe, hoʻopaʻa pane, dNTP
4	SARS-CoV-2hui ʻana o ka enzyme	80 μL / paipu	1 paipu	160 μL / paipu	1 paipu	Hoʻomaka wela Taq enzyme,M-MLV enzyme
5	SARS-CoV-2mana maikaʻi	100 μL / paipu	1 paipu	100 μL / paipu	1 paipu	ʻO ka plasmid recombinant i loaʻa ka pahuhopuʻāpana, RNA
6	SARS-CoV-2hoʻomalu ʻino	1200 μL / paipu	1 paipu	1200 μL / paipu	1 paipu	TE pale