Pūnaehana ʻIke DNA Foregene 20A (Hoʻoiho DNA manuahi)
ʻO ka wehewehe ʻana o ka pahu:
Hoʻohana ʻo Foregene DNA identification system 20A i ka ʻenehana hōʻailona fluorescent ʻelima kala e hoʻonui i ka 19 STR loci a me 1 sex loci i loko o ka paipu hoʻokahi.ʻOiai e uhi ana i 13 CODIS core loci, 20 core loci e hoʻokō i nā koi o ka Ministry of Public Security no ka autosomal STR kit.ʻO ia ka lima ʻākau o ke kūkulu ʻana i ka ʻikepili forensic DNA a me ka ʻike pili pili pili kānāwai.
wehewehe
Hoʻohana ʻo Foregene DNA identification system 20A i ka ʻenehana hōʻailona fluorescent ʻelima kala e hoʻonui i ka 19 STR loci a me 1 sex loci i loko o ka paipu hoʻokahi.ʻOiai e uhi ana i 13 CODIS core loci, 20 core loci e hoʻokō i nā koi o ka Ministry of Public Security no ka autosomal STR kit.ʻO ia ka lima ʻākau o ke kūkulu ʻana i ka ʻikepili forensic DNA a me ka ʻike pili pili pili kānāwai.
Kiko maʻiʻo
| Hui Hui | Inoa Hui | Volume（μl/tube） | Ka nui (tube) |
| PCR Pre-rection reagent | 2.5×PCR hoʻopaʻa paʻa Ⅲ | 1000 | 2 |
| 5×20A hui mua | 500 | 2 | |
| Kāohi DNA 9947A（1ng/μl） | 25 | 1 | |
| wai deionized | 1700 | 2 | |
| Taq Polymerase | Taq polymerase Ⅲ | 40 | 2 |
| PCR Rear-reaction reagent | Alapii Allelic20A | 40 | 1 |
| ORG 500 ke kaumaha o loko | 150 | 2 | |
| Nānā | |||
ʻIkepili ʻikepili
1. E wehe i ka polokalamu Genemapper ID.I ka hoʻohana ʻana i kēia pahu no ka manawa mua, pono ʻoe e hoʻokomo i nā panela, bin set, ke ʻano loiloi kūpono, a me ka maʻamau nui (ORG500: 65, 70, 80, 100, 120, 140, 160, 180, 200, 225, 250 , 275, 30, 30, 30, 30, 30, 30 0, 490, 500)
2. E hoʻokomo i ka ʻikepili electrophoresis, e koho i nā ʻāpana hoʻohālikelike e like me ka panel, ke ʻano o ka nānā ʻana, a me ka maʻamau o ka nui, a hoʻololi i ke ʻano hāpana o Ladder i "Allelic Ladder" ma ke kolamu "Sample Type";hoʻomaka i ke kālailai ʻikepili.
Waihona Reagent
1. Ma hope o ka loaʻa ʻana o ka pahu hau i loko o ka hau maloʻo a i ʻole nā pahu hau plastik, inā ʻaʻole hoʻohana ʻia no ka manawa pōkole, e ʻoluʻolu e mālama iā ia ma lalo o -20 ° C no ka manawa lōʻihi.
2. Ma hope o ka lawe ʻia ʻana o ka pahu i waho a hoʻohana ʻia, pono e mālama ʻia ka kit ma 4 ° C ma mua o ka hopena e pale aku ai i ka maloʻo a me ka hoʻoheheʻe ʻana;Pono e mālama ʻia ka enzyme Taq ma -20°C.
3. Ma hope o ka pane ʻana, pono e mālama ʻia ka pahu (me ke kaumaha o ka molecular paona kūloko a me ke alapiʻi) i loko o ka "keʻena ʻike electrophoresis" ma 4 ° C, e pale i ka hau a me ka hoʻoheheʻe ʻana, a pale aku i ka pili ʻana me ka kit pre-rection e pale i ka contamination.
