Pūnaehana ʻIke DNA Foregene 25A-C (Case)
ʻO ka wehewehe ʻana o ka pahu:
Hoʻohana ʻo Foregene DNA Identification System 25A-C i ka ʻenehana hōʻailona fluorescence ʻeono kala e hoʻonui i 23 autosomal STR loci, hoʻokahi Amel loci, hoʻokahi Yindel loci a me ʻelua loci hoʻomalu maikaʻi kūloko i ka manawa no ka hoʻonui ʻana i nā mamana DNA i unuhi ʻia a hoʻomaʻemaʻe ʻia mai nā laʻana hihia.
wehewehe
Hoʻohana ʻo Foregene DNA Identification System 25A-C i ka ʻenehana hōʻailona fluorescence ʻeono kala e hoʻonui i 23 autosomal STR loci, hoʻokahi Amel loci, hoʻokahi Yindel loci a me ʻelua loci hoʻomalu maikaʻi kūloko i ka manawa no ka hoʻonui ʻana i nā mamana DNA i unuhi ʻia a hoʻomaʻemaʻe ʻia mai nā laʻana hihia.
Kiko maʻiʻo
Hoʻomākaukau ʻōnaehana hoʻonui
ʻO ka hoʻomākaukau ʻana o ka ʻōnaehana unuhi maʻamau a hoʻonui
| Nā ʻāpana | 10μl ʻōnaehana (μl) | |
| Master Mix | 2.5×PCR Premix C Ⅰ | 4.0 |
| 10×25A-CPrimer Mix | 1.0 | |
| DNA template | 1—5 | |
| wai deionized | Hana i ka nui pane o 10μl |
ʻIkepili ʻikepili
Pākuʻi 1:Alapiʻi genotyping o allele
Pākuʻi 2:DNA type type 9948 typepalapala ʻāina
Waihona Reagent
1. Ma hope o ka loaʻa ʻana o ka pahu, e ʻoluʻolu e mālama iā ia ma lalo -20 ℃ inā ʻaʻole ʻoe e hoʻohana ia mea no ka manawa.
2. Ma hope o ka lawe ʻia ʻana o ka pahu a hoʻohana ʻia, mālama ʻia ka ʻāpana pre-reaction PCR premixed solution ma -20 ℃, a mālama ʻia nā reagent pre-reaction i koe ma 4 ℃ e pale i ka hoʻoheheʻe hou ʻana.Inā liʻiliʻi ka mea hoʻokahi, ʻōlelo ʻia e mālama ma -20 ° C ma hope o ka hoʻopili ʻana.
3. Ma hope o ka hopena, mālama ʻia nā ʻāpana ma 4 ℃ e pale i ka hoʻoheheʻe hou ʻana.Mai hoʻopā i nā reagents pre-reaction e pale i ka pollution.
