Foregene DNA Identification System 27Y
ʻO ka wehewehe ʻana o ka pahu:
Foregene Hoʻohana ʻo DNA Identification System 27Y i ka ʻenehana hōʻailona fluorescent ʻeono kala e hoʻonui i 27 Y-STR loci i ka manawa hoʻokahi.Me: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS537S3, DYS533, DYS393, DYS393 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
Hiki ke hoʻonui i ka pepa kānana, kāleka FTA, pulupulu, kāleka kohu, waha waha, a hiki ke hoʻohana ʻia e unuhi a hoʻomaʻemaʻe i nā palapala DNA mai nā mea hihia.
wehewehe
Foregene Hoʻohana ʻo DNA Identification System 27Y i ka ʻenehana hōʻailona fluorescent ʻeono kala e hoʻonui i 27 Y-STR loci i ka manawa hoʻokahi.Me: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS537S3, DYS533, DYS393, DYS393 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
Hiki ke hoʻonui i ka pepa kānana, kāleka FTA, pulupulu, kāleka kohu, waha waha, a hiki ke hoʻohana ʻia e unuhi a hoʻomaʻemaʻe i nā palapala DNA mai nā mea hihia.
Kiko maʻiʻo
| pahu ho'āʻo reagent | inoa mea | Ka nui (μl/piu) | Ka nui (paipu) |
| PCR pre-rection kit | 2×PCR Reaction Master Mix | 1250 | 2 |
| 5 x 27Y Hui Pūmua | 500 | 2 | |
| ʻO ke kūlana paʻi ʻana o DNA 9948 | 20 | 2 | |
| wai deionized | 1700 | 2 | |
| ʻO ka pahu hana ma hope o ka PCR | 27Y hui allele Alapiʻi | 20 | 2 |
| ORG500 paona o loko o loko o ke kaumaha molekala ʻalani | 150 | 2 |
ʻIkepili ʻikepili
1. E wehe i ka polokalamu Gene Mapper ID.I ka hoʻohana ʻana i kēia kit no ka manawa mua, pono ʻoe e hoʻokomo i nā panela, bin set, ke ʻano loiloi kūpono, a me ka maʻamau nui (ORG500: 65, 70, 80, 100, 120, 140, 160, 180, 200, 225, 250, 275, 30, 30, 30, 30, 30 , 490, 500).
2. E hoʻokomo i ka ʻikepili electrophoresis, e koho i nā ʻāpana hoʻohālikelike e like me ka panel, ke ʻano o ka nānā ʻana, a me ka maʻamau o ka nui, a hoʻololi i ke ʻano hāpana o Ladder i "Allelic Ladder" ma ke kolamu "Sample Type";e hoʻomaka i ke kālailai ʻana i ka ʻikepili.
Waihona Reagent
1. E ʻoluʻolu e mālama iā lākou ma lalo o -20 ° C (inā ʻaʻole hoʻohana ʻia no ka manawa pōkole), ma hope o ka loaʻa ʻana o nā pahu hau i ka hau maloʻo a i ʻole nā pahu hau gel.
2. E ʻoluʻolu e mālama i ka pahu ma 4°C ma mua o ka hoʻololi ʻana i mea e pale ai i ka maloʻo mau a me ka hoʻoheheʻe ʻana, ma hope o ka lawe ʻia ʻana o ka pahu no ka hoʻohana ʻana, ;Pono e mālama ʻia ka enzyme ma -20 ° C.
2. Ma hope o ka pane ʻana, pono e mālama ʻia ka pahu (me ke kaumaha o ka molecular paona kūloko a me ke alapiʻi) i loko o ka "keʻena ʻike electrophoresis" ma 4 ° C, e pale i ka hoʻoheheʻe pinepine ʻana a me ka hoʻoheheʻe ʻana, a pale i ka hoʻopili ʻana me ka kit pre-rection e pale i ka contamination.
