Foregene DNA Identification System 30A (Non-Extraction)
ʻO ka wehewehe ʻana o ka pahu:
Hoʻohana ʻo FOREGENE DNA identification system 30A i ka ʻenehana hōʻailona fluorescent ʻeono kala ihoʻonui 29autosomalSTR wahi, ʻekahiAmel locus a hoʻokahi Yindel locus i ka manawa hoʻokahi, a hiki ke hoʻonui pololei i ka pepa kānana, FTAkāleka, pulupulu pulupulu, kāleka kohu,a me ka mʻauʻau waho.
wehewehe
Hoʻohana ʻo FOREGENE DNA identification system 30A i ka ʻenehana hōʻailona fluorescent ʻeono kala ihoʻonui 29autosomalSTR wahi, ʻekahiAmel locus a hoʻokahi Yindel locus i ka manawa hoʻokahi, a hiki ke hoʻonui pololei i ka pepa kānana, FTAkāleka, pulupulu pulupulu, kāleka kohu,a me ka mʻauʻau waho.
Kiko maʻiʻo
Hoʻomākaukau Pūnaehana Amplification
Papa 1: ʻO ka hoʻomākaukau ʻana o ka hana hoʻonui maʻamau (Extraction-free).
|
| Cnā mea hoʻokūkū | 25 ʻōnaehana μl (μl ) | 10 ʻōnaehana μl (μl ) |
| Master Mix | 4 × Master Mix VII | 6.25 | 2.5 |
| 5 x 30AHui Pūmua | 5.0 | 2 | |
| wai deionized | 13.75 | 5.5 | |
| Blood lepo | 1.2mm anawaena | 1.2mm anawaena | |
| Total reaction volume | 25 μl | 10 μl | |
ʻIkepili ʻikepili
Pākuʻi 1:Palapala ʻAi Allelic Ladder Type
Pākuʻi 2:DNA type type 9948 typepalapala ʻāina
Waihona Reagent
1. Eʻoluʻolu e mālama ma lalo -20 ° C,, ma hope o ka loaʻa ʻana o nā pahu hau i ka hau maloʻo a i ʻole nā pahu hau gel.
2. E ʻoluʻolu, e mālama i ka mea hana mua 4×premix VII ma -20°C, ma hope o ka lawe ʻia ʻana o ka pahu no ka hoʻohana ʻana, a e mālama i nā mea hoʻonā hou i koe ma 4°C e pale i ka maloʻo a me ka hoʻolulu.Inā liʻiliʻi ka mea hoʻokahi, pono ia e mālama i ka -20 ° C ma hope o ka ʻai ʻana,
3. E mālama i nā ʻāpana ma hope o ka pane ʻana ma 4 ° C, e pale i ka maloʻo a me ka hoʻoheheʻe pinepine ʻana, a mai hoʻopā i nā reagents ma mua o ka hopena e pale ai i ka haumia.
