Nūhou ʻOihana
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Primer design-detection specificity, stability and amplification efficiency
ʻO ke kikoʻī o ka ʻike ʻana I ka nui o nā hihia, ʻo ke kumu o ka hoʻolālā mua e hoʻonui i ka kikoʻī o PCR.Hoʻoholo ʻia kēia e ka ʻoi aku a i ʻole ka liʻiliʻi o ka manaʻo o nā ʻano like ʻole.ʻO kekahi hoʻololi koʻikoʻi ʻo ia ke kaʻina ma ka 3′hopena o ka primer.ʻO ka mea nui, ua hoʻolālā ʻia ka PCR assays no ...Heluhelu hou aku -
Ua komo nā lāʻau nucleic acid i ka makahiki gula, he aha nā ʻenehana nui e pono e hoʻoholo koke ʻia?
Source: Medical Micro Ma hope o ka laha ʻana o COVID-19, ua ʻae koke ʻia nā lāʻau lapaʻau mRNA ʻelua no ke kūʻai aku ʻana, a ua ʻoi aku ka nui o ka nānā ʻana i ka hoʻomohala ʻana i nā lāʻau lapaʻau nucleic acid.I nā makahiki i hala iho nei, ua paʻi ʻia kekahi mau lāʻau nucleic acid i hiki ke lilo i mau lāʻau blockbuster ...Heluhelu hou aku -
Ka Ikepili o na Papa kuhikuhi Hooia o Primer Probes i ka manawa mua o PCR Reagents
ʻO ka hōʻoia ʻana i ka hana o nā primers a me nā probes i ka wā mua o ka PCR reagents a me ka hoʻoholo ʻana i nā kūlana hopena kūpono loa nā mea e pono ai e hōʻoia i ka holomua o nā hoʻokolohua maʻamau.No laila pehea mākou e pono ai e hōʻoia i ka noiʻi mua i ka wā mua ...Heluhelu hou aku -
ʻO ka laʻana i nā wahi ʻike pono e ʻike ʻia e nā mea nānā
Hoʻomaka ka hoʻāʻo ʻana o ke keʻena me ka hōʻiliʻili laʻana, a ʻo ka hōʻiliʻili laʻana ka mea maʻalahi ke nānā aku.ʻO ka mea nui loa no ka hōʻiliʻili laʻana, ʻo ia ke koho ʻana i ke ʻano laʻana kūpono, hoʻohana i nā mea hana laʻana kūpono, a hoʻokō i ka halihali a me ka hana kūpono.I.Sample type Common sam...Heluhelu hou aku -
ʻO ka hopena hope loa i ka pollution aerosol acid nucleic
ʻO nā ʻano PCR a me ka hoʻohaumia ʻana o ka waika nucleic aerosol i loko o nā hale hana hoʻāʻo nucleic acid e like me nā ʻaoʻao ʻelua o ke kālā.Hiki iā mākou ke koho e loaʻa a ʻaʻole paha, akā ʻaʻole hiki iā mākou ke koho inā makemake mākou a hoʻolilo paha.1. Ka nānā ʻana i ka mea wehe DNA No ka hoʻokō ʻana i ka wehe ʻana i ka spatial ...Heluhelu hou aku -
ʻIke wikiwiki i nā mea kanu transgenic
Ma ke ʻano he mea hou i loko o ka hale hoʻokolohua, ʻaʻole ia he hana maikaʻi e nānā i nā mea kanu maikaʻi mai kahi pūʻulu o nā mea kanu me ka haʻahaʻa haʻahaʻa.ʻO ka mea mua, pono e unuhi ʻia ka DNA mai ka nui o nā laʻana, a laila e ʻike ʻia nā genes haole e ka PCR.Eia naʻe, ʻaʻole pinepine nā hopena ...Heluhelu hou aku -
ʻO ka wehewehe piha ʻana i nā mea nui o ka SARS-CoV-2 nucleic acid test, no ke aha i loaʻa ai nā hewa hewa a hoʻopaʻa hou i nā hopena?
I ka wā mua o ka laha, ma muli o ka ulu wikiwiki ʻana, ʻo ka maʻi wikiwiki o nā mea maʻi i manaʻo ʻia ke kī i ka pale ʻana iā COVID-19.Kekahi mau nucleic acid detection reagents he manawa hoʻomohala pōkole, a aia nā pilikia e like me ka wikiwiki o ka hōʻoia ʻana i ka hana, ʻaʻole lawa ...Heluhelu hou aku -
He aha ka SNP?Nā kumuhana e pili ana i ka heluna kanaka
Loaʻa nā leka ʻekolu SNP i ke aʻo ʻana i ka genetics heluna kanaka.Ma waho o ka noiʻi ʻana i nā maʻi kanaka, ka hoʻonohonoho ʻana i nā ʻano mea kanu, ka ulu ʻana o nā holoholona a me ka molecular ecology, pono nā SNP i kumu.Eia nō naʻe, inā ʻaʻole ʻoe i ʻike hohonu i ke ʻano genetic hou...Heluhelu hou aku -
Pehea e hoʻomaikaʻi ai i ka pono o ka LncRNA reverse transcription?
Nā hiʻohiʻona lncRNA: 1. ʻOi aku ka lōʻihi o nā lncRNA, me ka hōʻike ikaika a me nā ʻano splicing like ʻole i ka wā o ka ʻokoʻa;2. Hoʻohālikelike ʻia me nā genes coding, ʻoi aku ka haʻahaʻa o ka lncRNA;3. Loaʻa ka hapa nui o nā lncRNA i nā kikoʻī kikoʻī o ke kino a me ka spatial i ke kaʻina hana ...Heluhelu hou aku -
ʻEhā Manaʻo nui no ka PCR Product Pollution Control
1: E hoʻololi i nā lako hoʻokolohua i ka manawa Set up (NTC) mana maikaʻi ʻole a hana hou i nā manawa he nui.I ka manawa e ʻike ʻia ai aia ka hoʻohaumia ʻana o ka huahana PCR i loko o ka hale hana, e hoʻololi i nā lako hoʻokolohua āpau i ka manawa.E like me: hoʻoheheʻe hou a hoʻomākaukau i nā primers, hoʻomaʻemaʻe hou i ka piko pipette, E...Heluhelu hou aku -
ʻElua mau hana ʻelua RT-PCR enzymes
ʻAʻole hiki i nā transcriptases hoʻohuli kuʻuna ke ʻae i nā wela kiʻekiʻe (ʻo ka mahana maikaʻi loa no ka hana MMLV ʻo 37-50 ° C, a ʻo AMV ʻo 42-60 ° C).ʻAʻole hiki ke hoʻohuli maikaʻi ʻia ka RNA viral paʻakikī i ka cDNA i nā haʻahaʻa haʻahaʻa, e hopena i ka ʻike pono ʻana.Tra...Heluhelu hou aku -
ʻenehana hoʻonui isothermal acid nucleic
ʻO ka PCR ka ʻenehana hoʻonui nui o ka nucleic acid a hoʻohana nui ʻia ma muli o kona naʻau a me kāna kikoʻī.Eia nō naʻe, koi ʻo PCR i ka denaturation thermal hou a ʻaʻole hiki ke hoʻopau i nā palena o ka hilinaʻi ʻana i nā mea kani a me nā mea hana, e kaupalena ana i kāna noi ʻana i ka lāʻau lapaʻau ...Heluhelu hou aku
